中国药业2012,Vol.21Issue(9):24-25,2.
高效液相色谱法测定金钱草颗粒中槲皮素含量
Content Determination of Quercetin in Lysimachia Granules by HPLC
谭忠谋 1韦建乔 1蒙露露1
作者信息
- 1. 广西壮族自治区河池市食品药品检验所,广西河池,574000
- 折叠
摘要
Abstract
Objective To establish an HPLC method for determining the content of quercetin in Lysimachia Granules. Methods HPLC method was used. The column type was Phenomenex Prodigy ODS3 (250 mm ×4. 6 mm, 5 μm).The mobile phase was methanol -0. 4% phosphorous acid(50 : 50). The flow rate was 1 mL/min. The detective wavelength was 360 nm and column tempreture was 30 ℃. Results The linear range of quercetin was 10. 4 - 104. 0 μg/mL(r = 0. 999 9), the average recovery was 99.92% and the RSD was 1.07%(n = 6). Conclusion The method is simple and accurate,and can be used reliably for the quality control of Lysimachia Granules.关键词
含量测定/金钱草颗粒/槲皮素/高效液相色谱法Key words
content determination/Lysimachia Granules/quercetin/HPLC分类
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谭忠谋,韦建乔,蒙露露..高效液相色谱法测定金钱草颗粒中槲皮素含量[J].中国药业,2012,21(9):24-25,2.