首页|期刊导航|中华医学杂志（英文版）|Role of baicalin in regulating Toll-like receptor 2/4 after ischemic neuronal injury
中华医学杂志（英文版）2012，Vol.125Issue(9)：1586-1593,8.DOI:10.3760/cma.i.issn.0366-6999.2012.09.012
Role of baicalin in regulating Toll-like receptor 2/4 after ischemic neuronal injury
Role of baicalin in regulating Toll-like receptor 2/4 after ischemic neuronal injury
摘要
Abstract
Background Baicalin has a significant anti-inflammation effect and is widely used in the clinical treatment of stroke.Most of the studies of Toll-like receptor 2/4 (TLR2/4) during cerebral ischemia had defined their specific expressions in microglia in hippocampus tissue.To explore the targets of baicalin in stroke,we detected the expressions of TLR2/4 in vitrolvivo.Methods By constructing a cerebral ischemia-reperfusion model in vivo and glucose oxygen deprivation model,we successfully induced neuron damage,then added baicalin and detected expressions of TLR2/4,nuclear factor-κB (NF-κB),tumor necrosis factor-alpha (TNFa),and interleukin-1β (IL-1β)in mRNA level and protein level.Results We found distinct upregulations of TLR2/4 and TNFα in both mRNA level and protein level in PC12 cells and primary neurons.Moreover,TLR2/4 and TNFα expressions were significantly higher in mice hippocampus treated with cerebral ischemia-reperfusion.Baicalin could downregulate the expressions of TLR2/4 and TNFα in the damaged cells and mice hippocampus effectively.Conclusions Neurons could respond to the damage and activate the related signal pathway directly.TLR2/4responsed to the damage and sent the signal to downstream factor TNFα through activating NF-κB.Baicalin could inhibit the inflammatory reaction in neuron damage and TLR might be its targets,which explained why baicalin could widely be used in the clinical treatment of stroke.关键词
Toll-like receptor 2/4/baicalin/neuron/oxygen glucose deprivation/cerebral ischemia-reperfusionKey words
Toll-like receptor 2/4/baicalin/neuron/oxygen glucose deprivation/cerebral ischemia-reperfusion引用本文复制引用
LI Hui-ying,YUAN Zhi-yi,WANG Yu-gang,WAN Hong-jiao,HU Jun,CHAI Yu-shuang,LEI Fan,XING Dong-ming,DU Li-jun..Role of baicalin in regulating Toll-like receptor 2/4 after ischemic neuronal injury[J].中华医学杂志（英文版）,2012,125(9):1586-1593,8.基金项目
Science Foundation of China (No.30973896,No.30801523,and No.81073092) and the National S&T Major Special Project for New Drug R&D Program of China (No.2009ZX09103-301,No.2009ZX09502,No.2009ZX09308-003,and No.2011ZX09101-002-11). （No.30973896,No.30801523,and No.81073092）
