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Discovery and identification of serum biomarkers of Wilms' tumor in mice using proteomics technology

JIA Zhan-kui WANG Jia-xiang YANG Jin-jian XUE Rui ZHANG Da WANG Guan-nan MA Sheng-li DUAN Zhen-feng

中华医学杂志（英文版）Issue(10)：1727-1732,6.

中华医学杂志（英文版）Issue(10)：1727-1732,6.DOI:10.3760/cma.j.issn.0366-6999.2012.10.009

Discovery and identification of serum biomarkers of Wilms' tumor in mice using proteomics technology

JIA Zhan-kui ¹WANG Jia-xiang ²YANG Jin-jian ¹XUE Rui ¹ZHANG Da ²WANG Guan-nan ³MA Sheng-li ⁴DUAN Zhen-feng⁵

作者信息

1. Department of Urologic Surgery , First Affiliated Hospital,Zhengzhou University, Zhengzhou, Henan 450052, China
2. Department of Pediatric Surgery , First Affiliated Hospital,Zhengzhou University, Zhengzhou, Henan 450052, China
3. Department of Pathology , First Affiliated Hospital,Zhengzhou University, Zhengzhou, Henan 450052, China
4. Department of Orthopedic Surgery, Second Hospital of Kaifeng City, Kaifeng, Henan 475002, China
5. Massachusetts General Hospital, Department of Hematology/Oncology, Boston 02138, USA
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摘要

Abstract

Background Wilms' tumor (nephroblastoma) is a cancer of the kidneys that occurs typically in children and rarely in adults.Early diagnosis is very important for the treatment and prognosis of the disease.The aim of our study was to discover and identify potential non-invasive and convenient biomarkers for the diagnosis of Wilms' tumor.Methods Nude mice were used to construct a Wilms' tumor model by injecting nephroblastoma cells into their bilateral abdomen.We collected 94 serum samples from mice consisting of 45 samples with Wilms' tumor and 49 controls.The serum proteomic profiles of the samples were analyzed via surface-enhanced laser desorption/ionization time-of-flight mass spectrometry.The candidate biomarkers were purified by high-performance liquid chromatography,identified by liquid chromatography-mass spectrometry,and validated using ProteinChip immunoassays.Results We finally retrieved two differential proteins (m/z 4509.2; 6207.9),which were identified as apolipoprotein A-Ⅱ and polyubiquitin,respectively.The expression of apolipoprotein A-Ⅱ was higher in the Wilms' tumor group than in the control group (P＜0.01).By contrast,the expression of polyubiquitin was lower in the Wilms' tumor group than in the control group.Conclusion Apolipoprotein A-Ⅱ and polyubiquitin may be used as potential biomarkers for nephroblastoma in children,and the analysis of apolipoprotein A-Ⅱ may help diagnose and treat Wilms' tumor.

关键词

proteomics/Wilms'tumor/biomarker

Key words

proteomics/Wilms'tumor/biomarker

引用本文复制引用

JIA Zhan-kui,WANG Jia-xiang,YANG Jin-jian,XUE Rui,ZHANG Da,WANG Guan-nan,MA Sheng-li,DUAN Zhen-feng..Discovery and identification of serum biomarkers of Wilms' tumor in mice using proteomics technology[J].中华医学杂志（英文版）,2012,(10):1727-1732,6.

基金项目

This work was supported by grants from the National Natural Science Foundation of China (No.81071782) and the Institution of Higher Education Specialized Research Fund for Doctor (No.200804590004). （No.81071782）

中华医学杂志（英文版）

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ISSN：0366-6999

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