中华耳科学杂志2012，Vol.10Issue(1)：60-64,5.
豚鼠外周血单个核细胞与耳蜗组织中糖皮质激素体含量的相关性分析
Correlation between glucocorticoid receptor levels in peripheral blood mononuclear cells and that in cochlea in guinea pigs
摘要
Abstract
Objective To study correlation of glucocorticoid receptor (GR) contents between peripheral blood mononucle-ar cells (PBMC) and the cochlea in guinea pigs by measuring the expression of GR mRNA and GR protein and the effects of systemic injection of dexamethasone on the expression level. Methods Healthy guinea pigs (n=32) were randomly divided into two groups (dexamethasone treatment and control groups). Dexamethasone (S mg/ml, 10 mg/kg/day) was injected intraperitone-ally in the IS guinea pigs of the dexamethasone group for 7 continuous days, while same volume physiological saline was injected intraperitoneally in the 17 guinea pigs of the control group. PBMCs and bilateral cochlear tissue were collected. Real time PCR and Western blot were used to detect the expression level of GR mRNA and GR protein in PBMCs and cochlear tissues.The data were analyzed with the SPSS 16.0 software. Results GR mRNA and CR protein were expressed in both PBMCs and cochlear tissues. The expression levels of CR mRNA and GR protein increased significantly in the dexamethasone treatment group and showed close correlation between PBMCs and the cochlea .Conclusions The levels of CR mRNA and GR protein in the PBMC indirectly reflect those in the cochlea in guinea pigs.关键词
受体/糖皮质激素/外周血单个核细胞/耳蜗/豚鼠Key words
Receptors/Clucocorticoid/Peripheral blood mononuclear cell/Cochlea/Guinea pig分类
医药卫生引用本文复制引用
陆玲,张秀玲,杨秭莹,吴骎,戴艳红,佘万东..豚鼠外周血单个核细胞与耳蜗组织中糖皮质激素体含量的相关性分析[J].中华耳科学杂志,2012,10(1):60-64,5.基金项目
2009年江苏省“六大人才高峰”项目、南京医学科技发展项目(WKK09105). （WKK09105）
