中华耳科学杂志2012，Vol.10Issue(1)：74-79,6.
阿米卡星致聋大鼠鼓阶壁上皮超微结构的改变
Ultrastructural changes of epithelium on wall of scala tympani in rats with Amikacin induced hearing loss
摘要
Abstract
Objective To investigate ultrastructural changes of epithelium attached to the bony wall of scala tympani in rat cochleae after amikacin sulfate administration and the structural basis for migration of transplanted mouse embryonic stem cells. Methods Rats were randomly divided into an experiment group (n=20) and a control group(n=10).For rats in the experiment group, amikacin sulfate was injected hypodermically at a dose of 200 mg/kg/day for seven days, while rats in the control group received physiological saline of the same volume instead. The cochlea was removed on Od, 7d, 14, 21d and 28d after amikacin administration was finished. Cochlea on one randomly selected side was prepared for SEM observation and the other co-chlear for HE examination. Results Amikacin sulfate administred to rats induced hearing loss. Epithelia on the bony wall of scala tympani underwent serial ultrastructural changes, including inflammatory exudation and increase of the intercellular spaces, which recovered in a time dependent manner. Conclusion Besides injury and loss of the hair cells in the Organ of Corti, amikacin sulfate treatment can also induce ultrastructural changes on the bony wall of scala tympani which may be the structural base for transplanted stem cell migration from scala tympani in the cochlea. Our results show the time window for inner ear stem cell therapy after ototoxic drugs injury.关键词
氨基糖甙类抗生素/耳蜗/超微结构/药物性耳聋/干细胞移植Key words
Aminoglycoside antibiotics/Cochlea/Ultrastructure/Drug-induced deafness/Stem cell transplant引用本文复制引用
李登科,赵立东,孙建和,刘慧占,杨仕明..阿米卡星致聋大鼠鼓阶壁上皮超微结构的改变[J].中华耳科学杂志,2012,10(1):74-79,6.基金项目
国家973计划重大科学研究计划干细胞项目(2012CB967900) （2012CB967900）
国家973计划重大科学问题导向项目(2011CBA01000) （2011CBA01000）
国家863计划专题项目(2007AA02Z150) （2007AA02Z150）
国家自然科学基金重点项目(30730040). （30730040）
