中华耳科学杂志2012，Vol.10Issue(1)：108-112,5.
二甲基亚砜对在体耳蜗毛细胞的毒性作用
Cytotoxic effects of dimethyl sulphoxide on cochlear hair cells in vivo
摘要
Abstract
Objective To assess cytotoxic effects of dimethyl sulphoxide (DMSO) on cochlear hair cells in vivo. Methods Healthy 4-week-old SD rats (100 - 120 g, n=32) of either sex were randomly divided into four groups to be treated with artificial perilymph (control group) or DMSO at 0.1%, 1% or 5%. Auditory brainstem responses (ABR) were tested 1 day before and 7 days after drug administration to determine its effects on hearing. Changes of cochlear hair cells were examined under a confocal microscope 7 days after drug administration. Results In the control group, ABR threshold shift was < 5 dB in response to clicks and 4, 8 and 16 kHz tone pips,and 13 dB to 32 kHz tone pips. DMSO treatment at 0.1% caused little or no damage. However, at 1% and 5%, DMSO caused considerable loss of OHC with ABR threshold shifts > 15 dB at most frequencies and > 30dB at 32 kHz. At 5%, DMSO caused damage in both outer and inner hair cells. Conclusion DMSO-induced damage appears to be dose-dependent. Hair cell damage caused by DMSO increases towards the basal turn in the cochlea.关键词
DMSO/耳蜗/毛细胞Key words
DMSO/cochlear/hair cells分类
医药卫生引用本文复制引用
王海霞,郭维维,杨仕明,张榕..二甲基亚砜对在体耳蜗毛细胞的毒性作用[J].中华耳科学杂志,2012,10(1):108-112,5.基金项目
国家973计划重大科学问题导向项目(2011CBA01000) （2011CBA01000）
国家973计划重大科学研究计划干细胞项目(2012CB967900)联合资助. （2012CB967900）
