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一株溶藻弧菌噬菌体的生理特性研究

曾林 邱德全 谢警鸿 于鸽

广东海洋大学学报2012,Vol.32Issue(1):24-29,6.
广东海洋大学学报2012,Vol.32Issue(1):24-29,6.

一株溶藻弧菌噬菌体的生理特性研究

A Study of the Biochemical Characteristics of the Vibrio alginolyticus Bacteriophage

曾林 1邱德全 2谢警鸿 1于鸽1

作者信息

  • 1. 广东海洋大学水产学院,广东湛江524025
  • 2. 广东海洋大学广东省海洋开发研究中心,广东湛江524025
  • 折叠

摘要

Abstract

The physiological characteristics of a Vibrio alginolyticus Bacteriophage was studied and the Bacteriophage was isolated from market of aquatic products in Zhanjiang. The results showed that the diameter of Bacteriophage plaque was l-1.5mm (12h). Lytic activity of Bacteriophage lost within 30 min when Bacteriophage exposed to UV light (20W, 30cm). Bacteriophage were UV-sensitive. They had strong lytic activity at pH 8 -11, and the optimum pH value was 11. The lytic cycle of them was 65 min. They were sensitive as the ambient temperature was more than 65℃. They were tolerated with ether and chloroform. They were sensitive to antiviral drugs acyclovir. Routine test dilution(RTD) was 106. Optimal multiplicity of infection was 10.

关键词

溶藻弧菌/噬菌体/生理特性

Key words

Vibrio alginolyticus/ Bacteriophage/ Biochemical characteristic

分类

生物科学

引用本文复制引用

曾林,邱德全,谢警鸿,于鸽..一株溶藻弧菌噬菌体的生理特性研究[J].广东海洋大学学报,2012,32(1):24-29,6.

基金项目

广东省科学技术厅项目(2009B020309002, 2011B020307011) (2009B020309002, 2011B020307011)

广东省海洋渔业局科技项目(A201008B05,A201008E03) (A201008B05,A201008E03)

广东海洋大学学报

OACHSSCDCSTPCD

1673-9159

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