浙江医学2012，Vol.34Issue(9)：708-711,4.
血糖波动大鼠核因子кB的变化及其对胰岛β细胞凋亡的作用
Effects of blood glucose fluctuation on NF-кB expression and apoptosis of islet β cell in rats
摘要
Abstract
Objective To investigate the effects of blood glucose fluctuation on NF- k B expression and apoptosis of islet β cells in rats. Methods Diabetes mellitus (DM) was induced by injection of STZ (20 mg/kg) and high fat feed in rats; The sustaining hyperglycemia (SHG) model was induced by intraperitoneal injection of 25% glucose 3 times/day, then subcutaneous injection of novorapid (1U/100g) was given to induce the glucose fluctuation (3 times/day) model (IHG). The rats were divided into 4 groups: normal group, DM group, SHG group, and IHG group. Two weeks after models were set, the pancreas were taken from animals of each group. The MDA, SOD levels in pancreatic tissue were measured; TNF-α, COX-2, BAX, BCL-2 and NF- k B were detected by ELISA methods; the apoptosis of islet β cells was detected by TUNEL method. Results Compared to normal group, MDA, TNF-α, COX-2, BAX, NF- k B levels and apoptosis index of islet β cells were increased in DM,SHG and IHG groups, while SOD and BCL-2 decreased. Compared to DM group, MDA, BAX, NF- k B levels and apoptosis index of islet P cells were increased in SHG and IHG groups. Compare to SHG group, NF- k B and apoptosis index of islet β cell were higher and BCL-2/Bax was lower in IHG group. BCL-2/Bax was negatively correlated with NF- k B (r=-0.239,P<0.05). Conclusion Glucose fluctuation may induce apoptosis of islet β cells, which might be assαiated with oxidative stress, inflammatory reaction and resulting activation of NF- k B.关键词
血糖波动/糖尿病/胰岛β细胞/核转录因子KBKey words
Glucose fluctuation/Diabetes/Mellitus Islet β/Cells NF- kBβ引用本文复制引用
金启辉,王辉,朱延涛,寿旗扬,谷卫..血糖波动大鼠核因子кB的变化及其对胰岛β细胞凋亡的作用[J].浙江医学,2012,34(9):708-711,4.基金项目
浙江省教育厅资助项目(N20100138) （N20100138）
浙江省自然科学基金(Y2110738) （Y2110738）
