肿瘤防治研究2012，Vol.39Issue(3)：329-334,6.DOI:10.3971/j.issn.1000-8578.2012.03.021
IFN-γ基因多态性与HBV感染及原发性肝细胞癌易感性的研究
Study on Susceptibility of HBV Infection and Primary Hepatocellular Carcinoma with Gene Polymorphism of IFN-gamma
摘要
Abstract
Objective To explore the distribution of cytokines IFN-gamma gene ( - 1615C/T and + 5171 A/G)single nucleotide polymorphisms in Guangxi people,and the impact of hepatitis B virus (HBV)infec-tion and primary hepatocellular carcinoma ( HCC) occurrence. Methods A case-control study based on hospital was carried out and all the objects were frequency matched by 375 HCC patients - 377 HBV car-riers-406 healthy control. TaqMan MGB Real-Time fluorescence quantitative PCR technology was applied to detect the SNPs of the two loci. The distribution of the genotype and the interaction of gene-environment in the three groups were analyzed by Logistic regression model. The linkage disequilibrium and hap-lotype of IFN-gamma gene were analyzed. Results There was no significant statistically difference in the polymorphisms of - 1615C/T and + 5171A/G loci among the three groups (P>0. 05). There were gene-environment interactions in smoking,alcohol consumption,liver cancer related family history with IFN-gamma gene according to logistic regression analysis. Alcohol consumption combined - 1615 locus mutant gene G increased HBV infection risk(OR= 1. 72,95YoCI:1. 11~3.26). The two loci mutant genes combined with liver cancer related family history also enhanced HCC risk (OR:29. 24,52. 03,95%CI:6. 91 ~ 123. 6,7. 02 ~ 385. 4,respectively). - 1615C/T and + 517WG sites on IFN-gamma had linkage disequilibrium (D' = 0. 976, P = 2. 22 -16 ), but the haplotypes between HCC groups and the total controls ( HBV carriers and healthy control)had no significant statistically difference. Conclusion The mutant genes of - 1615C/T and + 5171A/G loci might not influence the occurrence of HCC and HBV infection directly in the population of Guangxi,however they enhanced the risk interacted with the environment risk factors.关键词
原发性肝细胞癌/HBV感染/IFN-γ/基因多态性Key words
Primary Hepatocellular Carcinoma/HBV infection/Interferon gamma/Gene polymorphism分类
医药卫生引用本文复制引用
柏桦,仇小强,刘顺,贝春华,曾小云,余红平..IFN-γ基因多态性与HBV感染及原发性肝细胞癌易感性的研究[J].肿瘤防治研究,2012,39(3):329-334,6.基金项目
国家自然科学基金资助项目(30860247) （30860247）
广西自然科学基金资助项目(0832017Z) （0832017Z）
广西科学研究与技术开发资助项目(桂科攻0993003D-4) （桂科攻0993003D-4）
