中南民族大学学报：自然科学版2012，Vol.31Issue(1)：42-46,5.
槟榔十三味丸对慢性应激抑郁模型大鼠行为学及下丘脑-垂体-肾上腺轴的影响观察
Effects of Betel Shisanwei Ingredients Pill on the Behavior and Hypothalamic-Pituitary-Adrenal Axis in Depressed Rats
摘要
Abstract
Objective： To study the anti-depression mechanisms of Betel Shisanwei ingredients pill,its effects on the behaviors and hypothalamic-pituitary-adrenal axis were observed in the chronic depressed rats model.Method： Forty male SD rats were randomly divided into four groups according to the sugar consumption test and body weight（10 rats in each group）： Normal control group,Model group,Fluoxetine group and Betel Shisanwei ingredients pill group.Except the normal control,the other groups were treated with the chronic unpredictable mild stress stimulation combined with lonely raising for 21 days.10mL·kg-1 drugs was given to the rats,once daily,continuously for 21 days.After 21 days,behavior changes of rats were detected through open-field test,and sugar consumption test and body weight were also measured.Radioimmunoassay was used to analyze the levels of corticotrophin releasing hormone（CRH） in hypothalamus,adrenocorticotropic hormone（ACTH） in pituitary gland and cortisol（CORT） in serum.Results： Compared with normal control group,the scores of horizontal activity and vertical activity,sugar consumption and weight in model group decreased significantly（P0.01orP0.05）;Compared with model group,Fluoxetine group and Betel Shisanwei ingredients pill group got significantly higher scores of horizontal activity and vertical activity,sugar consumption and weight（P0.01）.The results of Radioimmunoassay showed that CRH,ACTH and CORT was significantly increased of model group compared with that of normal control group（P0.05orP0.01）;The Fluoxetine and Betel Shisanwei ingredients pill decreased the level of CRH,ACTH and CORT（P0.05orP0.01）.Conclusions： Betel Shisanwei ingredients pill could improve the behavior of depressive rats,and its regulating effect on HPA axis is one of the mechanisms for treating depression.关键词
槟榔十三味丸/抑郁症/应激模型/行为学/下丘脑-垂体-肾上腺轴Key words
Betel Shisanwei ingredients pill/depression/stress model/behavior/hypothalamic-pituitary-adrenal axis分类
药学引用本文复制引用
佟海英,莲花,高玉峰,包伍叶,李彦军,王淳,王大伟,韩志强..槟榔十三味丸对慢性应激抑郁模型大鼠行为学及下丘脑-垂体-肾上腺轴的影响观察[J].中南民族大学学报：自然科学版,2012,31(1):42-46,5.基金项目
北京市中医药科技发展基金资助项目 （）
