中山大学学报（自然科学版）2012，Vol.51Issue(2)：77-85,9.
鲤IGF2b基因内含子的克隆、基因组序列分析及慢病毒载体构建
Clone of IGF2b Introns, Analysis of Its Genomic Sequence and Construction of Lentiviral Vector (Lenti-IGF2-IRES-EGF)in Common Carp (Cyprinus carpio L.)
摘要
Abstract
To explore the relationship between the IGF2b gene and growth traits, we cloned the introns of IGF2b in common carp (Cyprinus carpio) muscle and analysis its characters. Further more, the lentivi-ral vector (Lenti-IGF2-IRES-EGF) was constructed and its activity was also researched. The results showed that; the length of 5 173 bp DNA sequence of IGFlb (HM755899) was get, including 3 introns and 4 exons. Two CpG islands in the 3'untranslated region and (T) n repeat sequence in the 5'untranslated region were found. Besides these, the lentiviral vector (Lenti-IGF2b-IRES-EGF) was constructed successfully, which was cotransfected into 293T cells with the GFP expression. The higher expression of IGF2 was found in 293T cells by Real time PCR. These illustrated that these study may be lay on solid foundation for relationship between IGF2b genetic diversity, expression and common carp growth traits.关键词
鲤/IGF2b/内含子/慢病毒载体Key words
common carp/ IGF2b/ introns/ lentiviral vector分类
生物科学引用本文复制引用
苏胜彦,董在杰,袁新华,梁政远,曲疆奇,张建桥,刘伟,马良骁,徐跑..鲤IGF2b基因内含子的克隆、基因组序列分析及慢病毒载体构建[J].中山大学学报（自然科学版）,2012,51(2):77-85,9.基金项目
现代农业产业技术体系建设专项资金资助项目(CARS-46) （CARS-46）
国家“十二五”科技支撑计划专题资助项目(2012BAD26B02) （2012BAD26B02）
中央级公益性科研院所基本科研业务费专项资金资助项目(2009JBFB01) （2009JBFB01）
江苏省自然基金资助项目(BK2010164) （BK2010164）
