中山大学学报（医学科学版）2012，Vol.33Issue(2)：172-177,6.
纳米金-表阿霉素复合体的体外抗肿瘤作用
In Vitro Antitumor Effects of Epirubicin-nanogold Compounds
摘要
Abstract
[Objective] To observe the in vitro antitumor effects of epirubicin-nanogold compounds (EPI-AuNP). [Methods] EPI-AuNP was prepared by chemosynthesis and investigated using UV-Vis spectrophotometer, fluorescence studies, dynamic light scattering,and zeta potential. Human umbilical vein endothelial cells (HUVEC) and HepG2 cells were divided into 4 groups; AuNP treatment group, EPI treatment group, EPI-AuNP treatment group and control group. After seeded in 96-well plate and cultured for 24 h separately, HUVEC and HepG2 cells were treated with 200 μL of AuNP,EPI,EPI-AuNP,and serum-free medium, respectively. Inhibition effect of each group on the HUVEC and HepG2 cells was assessed using MTT colorimetric method. UV-Vis spectrophotometer was applied to detect the cells epirubicin accumulation of different groups. [Results] A red shift in the SPR band maxima in the EPI-AuNP spectrum (λmax-525 nm) as compared with the spectrum of AuNP alone (λmax~520 nm) ;The fluorescence intensity of EPI (100 mg /L) was (195.2 ± 7.5) and EPI-AuNP was (16.4 ± 5.0) ,P=0.000. The hydrodynamic diameter of AuNP was (14.34±0.75) nm while EPI-AuNP was (18.54± 1.84) nm. Meanwhile, the zeta potential of AuNP was (-21.19 ± 0.64) mV while EPI- AuNP was (-15.34 ± 0.72) mV,P<0.01. The HUVEC survival rate of EPI-AuNP treatment group [(21.29 ± 1.51)%] was lower than the EPI group [(29.25 ± 1.59)%]. The HepG2 cells survival rate of EPI-AuNP treatment group [(43.82 ± 2.21)%] was lower than the EPI group [ (71.10 ± 4.16)%], P<0.01. [Conclusions] EPI-AuNP has been synthesized and indicated enhanced drug potency in vitro by MTT assay.关键词
纳米金-表阿霉素复合体/HUVEC/HepG2细胞Key words
epirubicin-nanogold compounds/ HUVEC/ HepG2 cells分类
医药卫生引用本文复制引用
赵晓旭,潘运龙,胡杨志,覃莉,丁晖,巫青..纳米金-表阿霉素复合体的体外抗肿瘤作用[J].中山大学学报（医学科学版）,2012,33(2):172-177,6.基金项目
国家基础研究973计划项目(2010CB833603) （2010CB833603）
国家自然科学基金资助项目(30772131) （30772131）
广东省自然科学基金资助项目(8151063201000034) （8151063201000034）
