中山大学学报（医学科学版）2012，Vol.33Issue(2)：190-194,5.
哮喘患儿外周血RORC和Foxp3mRNA表达水平的变化及其意义
Expression and Significance of RORC and Foxp3 mRNA in Peripheral Blood Mononuclear Cells (PBMC) in Children with Bronchial Asthma
摘要
Abstract
[Objective] To investigate the mRNA expression and significance of RORC (the transcription factor of Thl7 cells) and Foxp3 (the transcription factor of Treg cells) in PBMC in children with bronchial asthma. [Methods] To detect the mRNA expressions of RORC and Foxp3 in PBMC by quantitative real-time PCR in 40 asthmatic children, including 20 children of acute attack group (age 8.5 ±2.3) , 20 children of remission group (age 9.2 ±1.8) and 10 children of healthy control (age 8.0 ± 1.6). To analyze the relationship between the RORC and Foxp3 mRNA expressions and the FEV1 of pulmonary function in asthmatic children. [Results] It was showed that RORC mRNA expressions in asthmatic children was higher in attack stage than in remission stage and in control group (5.41 ± 0.12 vs 3.33 ± 0.27 vs 3.78 ±0.13, P< 0.01). In contrast, Foxp3 mRNA in asthmatic children was lower in attack stage than in remission stage and in control group (3.34 ± 0.12 vs 4.43 ± 0.07 vs 4.13 ± 0.04, P< 0.01). However, there was no significant difference in the expression of both RORC and Foxp3 mRNA between asthmatic children in remission stage and healthy group (P > 0.05). The ratio of RORC/Foxp3 mRNA in PBMCs of asthmatic children in attack stage was significantly higher than those in remission stage and healthy controls (1.68 ± 0.09 vs 0.76 ± 0.06 vs 0.92 ± 0.04, P < 0.01). Pearson correlation analysis revealed that RORC mRNA was negatively correlated with FEV,, but Foxp3 mRNA was positively correlated with FEV,(r = -0.58 and 0.52, P < 0.01). [Conclusions] The imbalance of RORC/Foxp3 mRNA expression exists in asthmatic children, suggesting a potential role forThl7/Treg imbalance in the pathogenesis and reduction of lung function in asthmatic children.关键词
哮喘/RORC/FOXP3/Th17细胞/Treg细胞Key words
asthma/ RORC/ Foxp3/ Thl7 cells/ regulatory T cells分类
医药卫生引用本文复制引用
黄雪琼,檀卫平,麦贤弟,吴葆菁,蓝丹..哮喘患儿外周血RORC和Foxp3mRNA表达水平的变化及其意义[J].中山大学学报（医学科学版）,2012,33(2):190-194,5.基金项目
广东省自然科学基金(9451008901003013) （9451008901003013）
广东省科学事业费计划项目(2010B031600228) （2010B031600228）
