作物学报2012，Vol.38Issue(1)：71-79,9.DOI:10.3724/SP.J.1006.2012.00071
青稞Wx基因多态性与直链淀粉含量的关系
Relationship between Polymorphism of Wx Gene and Amylose Content in Hulless Barley
摘要
Abstract
Hulless barley (Hordeum vulgare L. Var. Nudum Hook, f.) is a typical crop in Tibetan Plateau in China. Starch, including amylopectin and amylose, is the main storage component in hulless barley, of which amylose is in low content or even absent in the endosperm of waxy barley. The Waxy gene (Wx) located on the short arm of chromosome 7H is a key gene responsible for amylose synthesis, and also acts as a favorable gene in breeding of hulless barley. However, it is difficult to select hulless barley germplasm with Wx due to lack of effective molecular markers. This study aimed to find out molecular markers applicable in the, identification of Wx gene. A total of 150 accessions of hulless barley were primarily tested by staining endosperm with I2-KI, and four accessions (IG107028, Puebla, Huzhushuangcaoren, and APM-HC1905) were found as waxy genotypes. The amylose contents (AC) of the 150 accessions, which were measured using dual-wave length spectrophotometry method, varied from 12.4% to 38.5% with an average of 26.0%. The amylose contents of the four waxy genotypes namely IG107028, Brachvtic, Kunlun 2, and Huzhushuangcaoren, were 12.4-18.6% with an average of 16.7%. No genotype without amylase was found. We selected 51 accessions with obvious difference in AC to validate the polymorphism of four pairs of SSR markers. Primers PI and P3 showed no polymorphism among the 51 accessions, and primer P4 exhibited four types of amplified product, and the fragment size positively correlated with amylose content. The P4 primer was designed according to the sequence of Wx gene on chromosome 7H of barley. Primer P4 can be used as an effective marker for selecting waxy hulless barley cultivars.关键词
青稞/Wx基因/直链淀粉含量/分子标记Key words
Hulless barley/ Wx gene/ Amylose content/ Molecular markers引用本文复制引用
吴昆仑,赵媛,迟德钊..青稞Wx基因多态性与直链淀粉含量的关系[J].作物学报,2012,38(1):71-79,9.基金项目
本研究由现代农业产业技术体系建设专项资金(CARS-05)和引进先进农业科学技术计划(948计划)项目(2010-Z29)资助. （CARS-05）
