作物学报2012，Vol.38Issue(1)：99-106,8.DOI:10.3724/SP.J.1006.2012.00099
水稻籽粒氮代谢几个关键酶对花后高温胁迫的响应及其与贮藏蛋白积累关系
Response of Some Key Enzyme Activities Involved in Nitrogen Metabolism to High Temperature at Filling Stage and Its Relation to Storage Protein Accumulation in Rice Grain
摘要
Abstract
The influence of high temperature after flowering period on the activities of some enzymes, including glutamine syn-thetase (GS), glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) in developing grains, and also its relation to grain storage protein and amino acid accumulations were investigated by using two eaily-indica rice cultivars grown under two temperature regimes of daily average temperature 32°C and 22°C, respectively, and expression responses of two GS isoform genes to temperature were detected by real-time fluorescence quantitative PCR(FQ-PCR). The results indicated that the effects of high temperature after flowering period on GOT and GPT activities showed the similar trend, with increasing at early grain filling stage and decreasing at late grain filling stage. However, the increase of total contents of crude protein and various amino acids under high temperature after flowering period were not always due to the enhancement of grain nitrogen transport capacity and protein synthesis ability. GS activity under high temperature was generally higher than that under temperature in the same period. GS2, one of the isoforms of GS gene, was highly expressed in rice endosperm. The expression levels of GS2 was even higher than that of GS1 in late grain filling stage, while high temperature stress regulated GS activity through changing GS1 and GS2 genes transcription level in grain.关键词
水稻/高温/氮代谢/酶活性/蛋白积累Key words
Rice (Oryza sativa L.)/ High temperature/ Nitrogen metabolism/ Enzyme activity/ Protein accumulation引用本文复制引用
曹珍珍,张其芳,韦克苏,杨卫丽,刘光快,程方民..水稻籽粒氮代谢几个关键酶对花后高温胁迫的响应及其与贮藏蛋白积累关系[J].作物学报,2012,38(1):99-106,8.基金项目
本研究由国家自然科学基金项目(30871488)和浙江省自然科学基金项目(Y307086)资助. （30871488）
