作物学报2012，Vol.38Issue(1)：168-173,6.DOI:10.3724/SP.J.1006.2012.00168
水稻叶尖早衰突变体lad的形态、生理分析与基因定位
Analysis of Phenotype and Physiology of Leaf Apex Dead Mutant(lad)in Rice and Mapping of Mutant Gene
摘要
Abstract
A new mutant of leaf apex dead, lad, was obtained from the rice (Oryza sativa L.) restorer line Jinhui 10, which was induced by ethyl methanesulfonate (EMS). The leaf apexes of the mutant appeared normal before the emergence of the 5th leaf. However, the apexes of all leaves except for the 5th leaf turned yellow and gradually died after the expansion of the 5th leaf. Thereafter, the apexes of newly expanding leaves all became yellow and dead gradually. The physiological and biochemical analysis showed that the lad mutant contained lower contents of chlorophyll and soluble protein compared with the wild type, and the activity of superoxide dismutase (SOD) was abnormal in lad mutant. Besides, the plant height, leaf length, grain number, and seed setting rate were significantly lower in the mutant than in the wide type. The result of genetic analysis showed that the premature senescence character was controlled by a single recessive nuclear gene. Using an F2 population derived from Xinong 1A × lad, the LAD gene was mapped between SSR markers SWU11-19 and SWU11-5 on chromosome 11, and the genetic distance was 13 cM. This gene was cosegregated with markers SWU11-25 and SWU11-27. These results provide a basis for fine mapping and map-based cloning of LAD gene, and the lad mutant might be used in the mechanism study of leaf premature senescence of rice.关键词
水稻/叶尖早衰死亡/基因定位/表型分析/生理分析Key words
Rice/ Leaf apex dead/ Gene mapping/ Phenotypic analysis/ Physiologic analysis引用本文复制引用
杜青,方立魁,桑贤春,凌英华,李云峰,杨正林,何光华,赵芳明..水稻叶尖早衰突变体lad的形态、生理分析与基因定位[J].作物学报,2012,38(1):168-173,6.基金项目
本研究由国家自然科学基金项目(31071072)和重庆市重大攻关项目(CSTC,2010AA1013)资助. （31071072）
