作物学报2012，Vol.38Issue(1)：186-189,4.DOI:10.3724/SP.J.1006.2012.00186
高效液相色谱-紫外法同时检测小麦中DON、15ACDON和3ACDON
Establishment of a HPLC-UV Method for Simultaneous Determination of DON,15ACDON, and 3ACDON in Wheat
摘要
Abstract
A HPLC-UV method was established to determine deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ACDON) and 15-acetyldeoxynivalenol (15-ACDON) in wheat. Wheat sample was extracted by water, and the extracting solution was precipitated with an equal volume of ethanol followed by solid phase extraction with Oasis HLB cartridge. Qualitative and quantitative analyses of DON, 15ACDON and 3ACDON were done by HPLC-UVD using gradient elution program with acetonitrile and 0.005% phosphoric acid solution. Results showed that at the linear range of 0.5-15.0 mg L-1, the recoveries were 89.8%, 93.4%, and 92.9%, respectively. The relative standard deviations were 2.2%, 2.0%, and 2.5%, respectively, and the detection limit levels were 12.2, 10.5, and 16.7 μg kg-1, respectively. The method was accurate and reproducible. Sample purification lessened interference peaks, and due to the use of organic solvents was greatly reduced, the cost was decreased. Therefore, the method was suitable for the large batch determination of type B trichothecenes in wheat.关键词
小麦/B型单端孢酶烯族毒素/高效液相色谱-紫外检测法Key words
Wheat/ B trichothecenes/ HPLC-UV引用本文复制引用
杨丹,耿志明,马鸿翔,姚金保,张旭,张平平,张鹏..高效液相色谱-紫外法同时检测小麦中DON、15ACDON和3ACDON[J].作物学报,2012,38(1):186-189,4.基金项目
本研究由江苏省农业科技自主创新基金项目[CX(10)128],现代农业产业技术体系项目(nycytx-03),国家科技部国际合作项目(2009DFA32020)和国家转基因生物新品种培育重大专项(2008ZX08002-001)资助. （10）
