作物学报2012，Vol.38Issue(4)：614-623,10.DOI:10.3724/SP.J.1006.2012.00614
大豆异地衍生重组自交系群体遗传图谱的构建及比较
Comparison of Two Genetic Maps of Soybean Constructed by RIL Populations Derived from Combinations of Peking×7605 under Two Ecological Sites
摘要
Abstract
Molecular linkage maps provide a powerful tool for the analysis of plant genome structure and function, and it is important to determine the effect of ecological environment on soybean genetic map construction. Two soybean recombinant inbred line (RIL) populations named "JN(RN)P7" and "NJ(RN)P7" were tested in this study, which were derived from Peking x 7605 at Jinan and Nanjing, respectively. One hundred and forty-five SSR markers and one morphologic marker (BSC) which had polymorphism between parents were screened out to construct two genetic linkage maps using JoinMap 4.0 software. And we obtained two soybean genetic maps, which contained 27 and 25 of the linkage groups, respectively. The total length of two genetic maps was 1 574.80 and 1 682.50 cM, and the average distance between markers was 13.58 and 15.72 cM, respectively. The length of linkage group varied from 17.30 to 127.40 cM for JN(RN)P7, and from 20.10 to 137.50 cM for NJ(RN)P7. Both genetic maps were homologous with the public genetic map. The two maps constructed in this study were mostly coincident with each other, while certain differences were observed too, which indicated that there were some genetic structure differences between the two populations caused by the selection effect in various environments.关键词
大豆/异地衍生重组自交系/遗传图谱/构建/真实差异Key words
Soybean/RIL populations/Ecological sites/Genetic maps/Genetic structure differences引用本文复制引用
洪雪娟,侯金锋,丁卉,李永春,盖钧镒,邢邯..大豆异地衍生重组自交系群体遗传图谱的构建及比较[J].作物学报,2012,38(4):614-623,10.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2009CB118400),国家公益性行业(农业)科研专项(nycytx-004)和江苏高校优势学科建设工程资助项目资助. （973计划）
