植物学报2012，Vol.47Issue(2)：141-148,8.DOI:10.3724/SP.J.1259.2012.00141
白皮松花粉发育过程中蛋白酶体活性及其分布的动态变化
Dynamic Changes in Activity and Distribution of Proteasome During Pollen Development of Pinus bungeana
摘要
Abstract
Although the proteasome pathway has long been considered one of the most important proteolytic systems in pollen development, the dynamic changes of proteasome activity and distribution during pollen development are largely unknown. We performed fluorogenic kinetic assays using fluorogenic substrate sLLVY-AMC to measure proteasome activity in Pinus bungeana pollen grains. Our experiment results revealed that proteasome activity increased from the uninucleate to 3-cell stage, but slightly decreased in mature pollen grains. Immunofluorescence labelling detected proteasome signals in the nuclei of pollen grains at all stages as well as in cytosol. Unequal distribution of proteasomes was observed in the two degradating prothallial cells. Our data indicate a link between pollen development and dynamic changes in activity and distribution of proteasome, and proteasome might take a part in the degradation of prothallial cells.关键词
白皮松/花粉/蛋白酶体/原叶细胞Key words
Pinus bungeana, pollen, proteasome, prothallial cells引用本文复制引用
江丽萍,董晓玲,李雪,高圆,盛仙永..白皮松花粉发育过程中蛋白酶体活性及其分布的动态变化[J].植物学报,2012,47(2):141-148,8.基金项目
国家自然科学基金(No.30700039)和北京市教育委员会科技计划面上项目(No.KM201210028017) （No.30700039）
