植物学报2012，Vol.47Issue(2)：155-161,7.DOI:10.3724/SP.J.1259.2012.00155
玉米花粉体外萌发方法改进及其对花粉介导转基因的作用
The Improvement of Maize Pollen In Vitro Germination Method and Its Role in Pollen-mediated Plant Genetic Transformation
摘要
Abstract
The technique of pollen-mediated plant genetic transformation assisted by ultrasonication was patented by the Biotechnology Research Center of the Shanxi Academy of Agricultural Sciences. Here, we further investigated the conditions for sampling, storage and treatment of maize (Zea mays L.) pollen and effects on in vitro germination of pollen with the method. The optimal sucrose concentration for pollen suspension solution differed for maize pollen collected from plants with different flowering time. We determined the storage time and condition of pollen and their effects on pollen germination after ultrasonication. These results would be useful for improving pollen vitality in genetic transformation and verifying the feasibility of this plant transformation technique. We discuss some key steps for conducting in vitro germination of maize pollen and related parameters to increase transformation efficiency of pollen-mediated plant genetic transformation.关键词
转基因/体外萌发/花粉/玉米/超声波Key words
genetic transformation, in vitro germination, pollen, maize, ultrasonication引用本文复制引用
崔贵梅,孙毅,郝曜山,杜建中,王亦学..玉米花粉体外萌发方法改进及其对花粉介导转基因的作用[J].植物学报,2012,47(2):155-161,7.基金项目
国家转基因生物新品种培育重大专项(No.2008ZX08003-001,No.2009ZX08003-017B) （No.2008ZX08003-001,No.2009ZX08003-017B）
