植物学报2012，Vol.47Issue(2)：162-170,9.DOI:10.3724/SP.J.1259.2012.00162
芝麻愈伤组织诱导与植株再生体系的建立
Establishment of Sesame Callus Induction and Shoot Regeneration System
摘要
Abstract
We examined the effect of genotypes, explant types, combinations and concentrations of growth regulators on sesame callus induction and shoot regeneration with the cultivars Sesamum indicum L, 2n=26; the wild species S. Ra-diatum, 2n=64, and S. Schinzianum, 2n=64; and their distant hybrid strain S. Schinzianum * S. Indicum. We established the technical system of sesame callus induction and shoot regeneration with high frequency. Among the growth regulators, BA and NAA are suitable for inducing compact green callus, which gives a high differentiation frequency. The optimal medium for sesame callus induction and differentiation was MS basal medium supplemented with 0.1 mgL~1 NAA, 2.0 mg-L~1 6-BA and 30 g-L~1 sucrose. With this medium, the ratios of the highest level of callus induction and differentiation were 97.50% and 94.02% respectively, which were obtained from wild sesame hypocotyl explants, with the corresponding frequencies of 40.60% and 8.16% for cultivar hypocotyl explants. For the immature embryo explants from the distant hybrids, we achieved the maximum ratios of callus induction (46.67%) and differentiation (89.29%) with this medium. These results form the basis for both germplasm conservation and transgene studies in sesame.关键词
愈伤组织/基因型/诱导/再生/芝麻Key words
callus, genotype, induction, regeneration, sesame引用本文复制引用
苗红梅,琚铭,魏利斌,马琴,张海洋..芝麻愈伤组织诱导与植株再生体系的建立[J].植物学报,2012,47(2):162-170,9.基金项目
国家重点基础研究发展计划(No.2011CB109304)和国家芝麻产业技术体系建设项目(No.CARS-15) （No.2011CB109304）
