植物研究2012，Vol.32Issue(2)：208-212,5.
7种川产淫羊藿属植物遗传多样性及亲缘关系的ISSR分析
Genetic Diversity and Relationship Among 7 Species of Genus Epimedium in Sichuan Revealed by ISSR Analysis
摘要
Abstract
In order to investigate the genetic diversity and relationship of genus Epimedium in Sichuan province, 7 species including 12 populations were used to study the genetic variation by the ISSR marker technique. The result showed that 194 DNA fragments among 12 populations were amplified using 23 reliable ISSR primers, among which 169 DNA bands were polymorphic (the percentage of polymorphic bands reached 87. 11% ). The genetic distance and similarity among 12 populations were 0. 298 0-0. 620 7 and 0. 653 2-0. 748 9, indicating variation of genetic diversity existed among different populations. The observed effective number of alleles ( Ne) , Nei' s gene diversity index (He) and Shannon' s information index (I) were 1. 534 2, 0. 314 4 and 0. 469 7, respectively, showing the geographical distribution was very obvious. The result of cluster analysis using the unweighted pairgroup method with arithmetic average (UPGMA) and Principal Component analysis (PCA) based on ISSR data both showed that these tested resources were divided into two main groups. In addition, the ISSR marker technique is suitable for the analysis of the genetic diversity and relationship of genus Epimedium in Sichuan.关键词
淫羊藿属/ISSR/遗传多样性/亲缘关系Key words
Epimedium/ISSR/genetic diversity/genetic relationship分类
农业科技引用本文复制引用
陈燕英,黎云祥,权秋梅,廖兴利,成仙鸽..7种川产淫羊藿属植物遗传多样性及亲缘关系的ISSR分析[J].植物研究,2012,32(2):208-212,5.基金项目
四川省教育厅项目(09ZX011) （09ZX011）
四川省科技厅应用基础研究项目(2008JY0158) （2008JY0158）
四川省重点实验室开放基金项目(XNYB0904) （XNYB0904）
