植物研究2012，Vol.32Issue(2)：227-231,5.
影响根癌农杆菌介导的木薯遗传转化因素分析
Analysis on the Factors Influencing Transformation of Cassava ( Manihot esculenta Crantz) Mediated by Agrobacterium tumefaciens
摘要
Abstract
Agrobacteriurn tumefaciens -mediated transformation system was used to study the factors influencing transformation in cassava. Histochemical GUS assay showed that the transient expression rate was significantly improved by using the following transformation conditions; GV3101 as A. Tumefaciens strains, infection time 30 -45 min, co-culture time 3 - 4 d, engineering bacterium concentration (OD600) 0.45 and application of 200 μmol · L-1 AS. However, pre-culture showed negative influence on the transient expression rate. Eight transgenic cassava plants from 453 explants were obtained in this study and the integration of glgC336 transgene was confirmed by PCR and Southern blot analysis. The transformation frequency was 1.77%.关键词
木薯/农杆菌介导/遗传转化Key words
cassava (Manihot esculenta Crantz)/agrobacterium-mediated/transformation分类
农业科技引用本文复制引用
姚庆荣,郭运玲,孔华,贺立卡,郭安平..影响根癌农杆菌介导的木薯遗传转化因素分析[J].植物研究,2012,32(2):227-231,5.基金项目
中国热带农业科学院科技基金项目(RKY0724) （RKY0724）
中国热带农业科学院中央公益性科研院所基本科研业务费专项(ITBBZD2007-2-3) （ITBBZD2007-2-3）
