植物营养与肥料学报2012，Vol.18Issue(2)：405-416,12.
叶面喷施尿素对葡萄氮代谢相关基因表达的影响
Effects of foliar applied urea on expression of genes related to nitrogen metabolism in Fujiminori grapevine
摘要
Abstract
Five important genes coding for nitrate reductase （NR）, nitrite reductase （NiR）, glutamine synthetase （GS）, glutamate dehydrogenase （GDH） and asparagine synthetase （AS）, were cloned from the related EST sequences deposited in VitisEST database and amplified using RT-PCR. Sub-cellular localization was employed to characterize their functional position, while semi-quantitative RT-PCR＇ and fluorescent quantitative PCR were both used to analyze their expression in leaves after treatment with urea at different concentrations. Results show that the five genes had significantly higher expression in younger leaves compared to old leaves. The differences in expression of these five genes at different times were inconsistent, but generally expression was increased by application of foliar urea, with application of 0. 3 % and 0. 5 % urea causing significant increases in gene expression compared to control plants. The expression of the five genes related to foliar fertilizer application was corroborated by the significant increases in related fruit indicators. Expression of GS in grape leaves had the most significant increase at 6 h after spraying of urea, while NR maintained a relatively high level of expression even at 48h after urea application compared to the other genes. NR and GS expression in various stages was higher than NiR and AS respectively, with the trends in expression of NiR and AS being consistent with those of NR and GS respectively. This study provides a theoretical basis for further studies on the mechanisms of nitrogen metabolic pathways and scientifically informed foliar fertilizer application.关键词
葡萄/氮代谢/基因克隆/亚细胞定位/基因表达Key words
grapevine/nitrogen metabolism/gene clone/sub-cellular localization/gene expression分类
农业科技引用本文复制引用
初建青,王文艳,房经贵,张春华,张彦平,宋长年..叶面喷施尿素对葡萄氮代谢相关基因表达的影响[J].植物营养与肥料学报,2012,18(2):405-416,12.基金项目
江苏省农业综合开发科技推广项目 （）
教育部新世纪优秀人才支持计划（NCET-08-0796）资助. （）
