植物营养与肥料学报2012，Vol.18Issue(2)：444-450,7.
接种自生固氮菌对玉米根际土壤酶活性和细菌群落功能多样性的影响
Effects of inoculating azotobacter on soil enzyme activities and bacterial community functional diversity in the rhizosphere of maize （Zea mays L. ）
摘要
Abstract
In order to explore the response of soil micro-ecological characteristics associated with the rhizosphere of maize （Zea mays L. ） to azotobacter＇ s inoculation, the responses of soil enzyme activities and bacterial community functional diversity in the rhizosphere soils of maize seedlings inoculated with azotobacter isolates including Azoto- bacter chroococcum YCYS, PhyUobacterium brasssicacearum QL54 and PaenibaciUus sabinae MX31 were examined in pot culture conditions. The results show that the inoculation of azotobacter can change the soil enzyme activities and regulate the soil bacterial functional community associated with the rhizosphere of maize, and different strains have different inoculation effects. Compared with the control, the urease activities of A. chroococcum YCYS and P. sabinae MX31 treatments are increased by 20. 55% and 9.58% , respectively. However, there are not significant differences between the inoculation and control treatments in the phosphatase activity （ P 〉 0.05 ）. The BIOLOG results show that the azotobacter inoculation can increase the total bacterial metabolic activity, the activity of inoculating A. chroococcum YCYS is 1.8 times higher than that of the control. Meanwhile, the species richness （R） is significantly higher that that of the control （P 〈0. 01 ）. The results of six carbon sources reveal that there are different carbon metabolic communities among the treatments. Principle components analysis （PCA） suggests that the azotobacter inoculation can regulate the bacterial community associated with the rhizosphere soils.关键词
自生固氮菌/玉米/BIOLOG/土壤酶活性/细菌群落功能多样性Key words
azotobacter/Zea mays/BIOLOG/soil enzyme activity/bacterial community functional diversity分类
农业科技引用本文复制引用
陈胜男,谷洁,付青霞,孙薇,钱勋,高华,秦清军..接种自生固氮菌对玉米根际土壤酶活性和细菌群落功能多样性的影响[J].植物营养与肥料学报,2012,18(2):444-450,7.基金项目
国家自然科学基金（40871119 （）
41171203） （）
农业部948项目（2010-Z20）资助. （）
