植物营养与肥料学报2012，Vol.18Issue(3)：689-698,10.
白籽南瓜嫁接对不同盐胁迫下黄瓜幼苗氮代谢和蛋白表达的影响
Effects of graft with pumpkin rootstock on nitrogen metabolism and protein expression in the cucumber seedlings under iso-osmotic Ca （ NO3 ） 2 or NaCI stress
摘要
Abstract
expression cucumber Effects of grafting on the growth, nitrogen metabolism, osmoregulatory substances and soluble protein was conducted with pumpkin （ Cucurbita maxima × Cucurbita moschata） as the rootstock and a cultivar ＇Jinyou No. 3＇ as the scion under iso-osmotic Ca （NO3 ）2 or NaC1 stress. Results showed that the rootstock-grafted seedlings significantly enhanced the growth, NR activity, NO3--N, soluble protein, soluble sugar, proline content and decreased NH＋4-N content under iso-osmotic Ca（ NO3 ）2 and NaC1 stress. SDS -PAGE profilesshowed that there were twelve differentially-expressed proteins in leaves of rootstock-grafted seedlings in present of salt treatment. Of these twelve proteins, ten proteins were up-regulated including the proteins of relative molecular weight （ ×10^3） for 188.4, 156. 1, 120. 4, 54. 0, 37.3, 32. 1, 30. 6, 18. 9, 17. 8 and 17. 1, while one proteins of 81.0× 10^3 were down-regulated and turned a novel protein of 63.4 x 103. And then, these twelve proteins were probably related to enhancement of salt-tolerance of rootstock-grafted cucumber seedlings. Results a/so showed theexpressing of four proteins of relative molecular weight （ ×10^3 ） such as 86. 1,23.4, 18. 9 and 17.8 were stronger in both self-grafted and rootstock-grafted seedlings under NaC1 stress than that under Ca （NO3）2 stress, whereas the expressing of proteins relative molecular weight （×10^3 ） such as 188. 4, 156. l, 54. 0 and 17. 1 were lower under NaC1 stress than that under Ca （NO3）2 stress. And three proteins disappeared in self-grafted seedlings under NaCl stress for 9 days related to the high tolerance to Ca（ NO3 ）2 stress of rootstock-grafted of 39. 1 ×10^3, 35.4×10^3 and 21.2×10^3 even It was possible that these eleven proteins were cucumber seedlings. In conclusion, the damages caused by NaC1 stress were more serious than that by Ca （ NO3 ）2 stress, and rootstock-grafting could effectively alleviated the injury by regulating nitrogen metabolism and osmoregulatory substances of cucumber seedlings under salt stress. It seemed that the alleriating effect in rootstock-grafted plants more expressing under Ca（ NO3 ）2 stress.关键词
嫁接黄瓜/白籽南瓜/NaCl/Ca（NO3）2/氮代谢/渗透调节物质/SDS-PAGEKey words
gratted cucumber/Gucurbita maxima × Cucurbita moschata/N aCl/Ca(NO3)2/nitrogen metabolism/osmoregulatory substances/SI)S - PAGE分类
农业科技引用本文复制引用
王丽萍,孙锦,郭世荣,田婧,阳燕士蜀..白籽南瓜嫁接对不同盐胁迫下黄瓜幼苗氮代谢和蛋白表达的影响[J].植物营养与肥料学报,2012,18(3):689-698,10.基金项目
国家重点基础研究发展计划项目 （）
国家自然科学基金项目 （）
现代农业产业技术体系建设专项 （）
教育部高校博士点基金项目 （）
江苏省高校优势学科建设工程项目 （）
江苏省农业三新工程项目[SX（2011）289]资助. （）
