中国中西医结合杂志2012，Vol.32Issue(3)：333-337,5.
基于高效液相色谱-飞行时间质谱技术的高血压病肝阳上亢证尿液代谢组学研究
Urine Metabonomic Study on Hypertension Patients of Ascendant Hyperactivity of Gan Yang Syndrome by High Performance Liquid Chromatography Coupled with Time of Flight Mass Spectrometry
摘要
Abstract
Objective To study the changes of urine metabolites in hypertension patients of ascendant hyperactivity of Gan yang syndrome (AHGYS), and to explore its essence in hypertension patients. Methods Ten typical hypertension patients of AHGYS were recruited as the patient group, and the other twelve healthy volunteers were recruited as the normal group. The metabolite profiling in the urine were collected using by high performance liquid chromatography coupled with time of flight mass spectrometry (HPLC-TOFMS). The principal component analysis (PCA) and partial least-square discriminant analysis (PLS-DA) were analyzed using SIM-CA-P Software. The differential metabolites in the urine were found out and identified. The possible relevant metabolic pathways were explained. Results The data from the analysis by PCA in the urine samples of the patient group and the normal group showed, two sets of data could be obviously classified in the score plot. Compared with the normal group, significant changes happened to the body metabolism in the patient group. The metabolites relevant to hypertension patients of AHGYS were determined using the PLS-DA. Fifteen compounds of the structure and metabolic pathways had been confirmed through inquiring KEGG Database, mainly including amino acids, free fatty acids, sphingosine, and so on. Conclusions The hypertension patients of AHGYS were studied using HPLC-TOFMS combined with pattern recognition, thus finding out small molecular metabolic markers from the microscopic field, which was advantageous in probing the biological nature of Chinese medicine syndromes.关键词
代谢组学/高效液相色谱-飞行时间质谱技术/模式识别/高血压病肝阳上亢证Key words
metabolomics/high performance liquid chromatography coupled with time of flight mass spectrometry/pattern recognition/hypertension of ascendant hyperactivity of Gan yang syndrome引用本文复制引用
蒋海强,李运伦,解君..基于高效液相色谱-飞行时间质谱技术的高血压病肝阳上亢证尿液代谢组学研究[J].中国中西医结合杂志,2012,32(3):333-337,5.基金项目
国家自然科学基金资助项目(No.30772865) （No.30772865）
教育部新世纪优秀人才支持计划资助项目(No.NCET-07-0522) （No.NCET-07-0522）
山东省优秀中青年科学家奖励基金资助项目(No.2008BS03029) （No.2008BS03029）
山东省2009-2010中医药科技发展计划资助项目(No.2009-018) （No.2009-018）
山东省“泰山学者”建设工程专项经费资助项目 （）
