中国中西医结合杂志2012，Vol.32Issue(3)：385-389,5.
葛根素对钴60-γ射线致小鼠睾丸支持细胞急性损伤的保护作用
Protective Effects of Puerarin on 60Co-γ-induced Acute Injury of sertoll Cells of Testis in Mice
摘要
Abstract
Objective To study the protective effects of puerarin on "Co-y-induced acute injury of sertoli cells of testis in mice and the mechanisms for its radiation protection. Methods Forty male Wistar mice were randomly divided into four groups, I. E., the normal group, the model group, the low dose puerarin group, and the high dose puerarin group, respectively, 10 in each. The lower abdomen and scrotal area of mice were irradiated with a single dose of 8 Gy 60Co-y ray for 517 s in the model and two puerarin groups. Twenty-four h after modeling, different concentrations of puerarin (at the daily dose of 230 and 460 mg/kg respectively) were given to mice in the low and high dose groups by gastrogavage, once daily for two successive weeks. Equal volume of physiological saline was given to mice in the normal group and the model group, once daily for two successive weeks. The morphology of the testicular tissues was observed. The levels of serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and inhibin β were determined by ELISA. The relative expressions of Inhibinp mRNA in the testicular tissues were determined by Real-time quantitative PCR. Results Sertoli cells and spermatogenic cells were injured at different degrees in the model group. They were improved in the low and high dose puerarin groups, more obviously in the high dose puerarin group. Compared with the normal group, the level of serum FSH in the model group was significantly improved, while the serum level of Inhibin p and the expression of Inhibin p mRNA obviously decreased, showing statistical difference (P<0.05). Compared with the model group, the levels of serum FSH, Inhibin β, and the expression of Inhibin (3 mRNA were significantly improved in the low and high dose puerarin groups (P<0.05), more obviously in the high dose puerarin group (P<0.01, P<0.05). Conclusion Chinese medicine monomer of puerarin had certain protective effects on 60Co-y-induced acute injury of Stetoli cells in mice.关键词
葛根素/钴60-γ/睾丸支持细胞Key words
puerarin/60Co-y/testicular sertoli cell引用本文复制引用
王瑞,王磊,张卫星,秦俊昌,张杰..葛根素对钴60-γ射线致小鼠睾丸支持细胞急性损伤的保护作用[J].中国中西医结合杂志,2012,32(3):385-389,5.基金项目
河南省科技攻关项目(No.102102310067) （No.102102310067）
