中医药信息2012，Vol.29Issue(2)：13-15,3.
莱菔子素对人肝细胞Ⅱ相代谢酶的作用研究
Induction of Phase Ⅱ Metabolic Enzymes by Sulforaphene in Human Immortalized Hepatocytes
摘要
Abstract
Objective: To study the effect of sulforaphene on phase II metabolic enzymes expressions in human immortalized hepatocytes ( HHL - 5 cells). Methods: MTT assay was used to test the cytotoxicity of sulforaphene. HO -1 and TrxR expressions were detected by western blot analysis. Results: Sulforaphene promoted HHL -5 cells growth at 5μmol/L and inhibited cells growth at 10 - 100μmol/L. Moreover, HO - 1 and TrxR proteins were up -regulated in a dose - dependent manner at 24h. Conclusion; Sulforaphene can induce the expressions of HO -1 and TrxR proteins, which are attributed to the detoxification functions of chemical carcinogen.关键词
莱菔子素/莱菔硫烷/血红素加氧酶-1/硫氧还蛋白还原酶Key words
Sulforaphene/Sulforaphane/Heme oxygenase - 1/Thioredoxin reductase分类
医药卫生引用本文复制引用
潘萌,黄睿,潭洁,李宝龙,冯雪,王凤前,单毓娟..莱菔子素对人肝细胞Ⅱ相代谢酶的作用研究[J].中医药信息,2012,29(2):13-15,3.基金项目
国家自然科学基金资助(81172648) （81172648）
