组织工程与重建外科杂志2012，Vol.8Issue(1)：8-13,6.DOI:10.3969/j.issn.1673-0364.2012.01.003
地塞米松对人牙髓细胞增殖分化影响的研究
Effect of Dexamethasone on Proliferation and Differentiation of Human Dental Pulp Cells In Vitro
邹慧儒 1张兰成 2秦宗长 1杨敏 1Steven BROOKES 1Xuebin YANG2
作者信息
- 1. 300041 天津市 南开大学附属口腔医院
- 2. Leeds LS2 9LU,UK.利兹大学口腔研究所
- 折叠
摘要
Abstract
Objective To investigate the effect of dexamethasone on proliferation and differentiation of human dental pulp cells in vitro. Methods Human dental pulp cells (hDPCs) were isolated from extracted healthy premolars and third molars for clinical reasons like orthodontics and impaction. The teeth were immediately cracked open, and the pulp tissue was dissected and digested by tissue-explant enzyme digestion. The fourth passage of hDPCs were used for experiment. The cells were randomly divided into two groups, experimental group and control group. The cells in the experimental group were treated with 10-8 mol/L Dexamethasone (Dex) in 2% FBS aMEM media. The cells in the control group were cultured in 2% FBS aMEM media only. At 1, 3 and 5 days after culture, quantitative determination of hDPCs proliferation activity was observed. At 1, 5 and 10 days after culture, Alkaline phosphatase (ALP) staining and quantitative measurement were examined histologically and biochemically. At 10 days after culture, Alizarin red staining was used to observe the calcified nodules. Results Cell proliferation detection: 10-8 mol/L Dex significantly inhibited cell proliferation of hDPCs at 3 and 5 days after culture. There were significant difference between experimental group and control group; ALP staining and quantitative analysis: quantitative analysis of ALP activity showed at 10 days after culture, Dex significantly enhanced ALP activity of HDPCs compared with control group; Alizarin Red staining results: At 10 days after culture, both groups showed positive staining, which indicated the calcified nodule formation in both groups. However, the number of calcified nodule accumulated in experimental group was higher than the control group. Conclusion Tissue-explant enzymatic digestion method could successfully cultivate hDPCs in vitro. Dexamethasone significantly inhibited human dental pulp cell proliferation. Conversely, Dexamethasone strongly stimulated alkaline phosphatase activity and promoted calcified nodule formation.关键词
人牙髓细胞/地塞米松/增殖/分化/碱性磷酸酶活性Key words
Human dental pulp cells/ Dexamethasone/ Proliferation/ Differentiation/ Alkaline phosphatase activity分类
生物科学引用本文复制引用
邹慧儒,张兰成,秦宗长,杨敏,Steven BROOKES,Xuebin YANG..地塞米松对人牙髓细胞增殖分化影响的研究[J].组织工程与重建外科杂志,2012,8(1):8-13,6.
