癌变·畸变·突变2012，Vol.24Issue(3)：205-208,212,5.DOI:10.3969/j.issn.1004-616x.2012.03.009
人胚肾细胞HEK293中miRNA-128-1表达调控机制的初步研究
The mechanism of miRNA-128-1 expression in HEK293 cells
徐可 1刘明发 1金涛 1蒋嵩山 1许海雄1
作者信息
- 1. 中山大学附属汕头市中心医院神经外科,广东汕头515000
- 折叠
摘要
Abstract
OBJECTIVE: To discuss mechanism of miRNA-128-1 expression in HEK293 cells and lay the foundation for further study of miRNA-128-1 regulation in glioma. METHODS: Through analyzing UCSC, we found the probable miRNA-128-1 promoter area. We used UCSC and CBIL to obtain the predicted transcription factors binding on the miRNA-128-1 promoter area. Related plasmids were constructed, including pB-miRNA-128-1 promoter, pShNFl, pShc-Myc, pShTAFl, pShYYl, pShMAF, pShRELA2, pShRNUXl and pShNFkappaB. Cotransfecting pB-miRNA-128-1 promoter and pShRNA into HEK293 cells, we analyzed luciferase activity to identify probable transcription factors which could regulate miRNA-128-1 promoter activity. RESULTS: pShc-Myc suppressed the activity of pB-miRNA-128-1 promoter. pShNFkappaB increased the activity of pB-miRNA-128-1 promoter dramatically. pShNFl, pShTAFl, pShYYl, pShMAF, pShRELA2 or pShRNUXl showed no obvious influence on the expression of pB-miRNA-128-1. CONCLUSION: In HEK 293 cells, c-Myc upregulated whilst NFkappaB downregulated the promoter activity of miRNA-128-1.关键词
miRNA-128-1/表达调控/人胚肾细胞Key words
miRNA-128-1/regulation of expression/HEK293 cells分类
生物科学引用本文复制引用
徐可,刘明发,金涛,蒋嵩山,许海雄..人胚肾细胞HEK293中miRNA-128-1表达调控机制的初步研究[J].癌变·畸变·突变,2012,24(3):205-208,212,5.
