癌变·畸变·突变2012，Vol.24Issue(3)：209-212,4.DOI:10.3969/j.issn.1004-616x.2012.03.010
稳定干扰SET肝细胞株的生物学鉴定
Biologic identification of SET protein-deficient L-02 cells
摘要
Abstract
OBJECTIVE: This study aimed to identify the biological characteristics of SET protein-deficient L-02 cells which could lay a foundation for the role of SET protein in normal L-02 cells. METHODS: Normal L-02 cells, SET protein-deficient L-02 cells (stably expressing SET siRNA), siRNAc transfected L-02 cell were cultured and the growth and the morphology of these three groups of cells were examined and analyzed. The growth curves of the cells were generated on the data from MTT assay. Chromosome structure was detected by chromosome aberration analysis. Malignant transformation was identified by soft agar cloning test. RESULTS: Compared with normal L-02 cells, no obvious changes of cytomorphology and growth curves were observed for SET protein-deficient L-02 cells and siRNAc transfected L-02 cells. Chromosome aberration analysis revealed that transfection of SET siRNA did not cause obvious changes in the structure and numbers of chromosomes in L-02 cells. Soft agar cloning test showed that transfection of SET siRNA did not cause malignant transformation of the cells. CONCLUSION: Transfection of SET siRNA did not significantly alter the biologic characteristics of the cells, indicating that the established SET-deficient cells can be used as a model for further studies.关键词
三氯乙烯/稳定干扰SET肝细胞/生物学性状/细胞增殖Key words
trichloroethylene/SET protein-deficient L-02 cells/biological characters/cell proliferation分类
生物科学引用本文复制引用
周丽,李杰,黄遂,杨细飞,刘建军..稳定干扰SET肝细胞株的生物学鉴定[J].癌变·畸变·突变,2012,24(3):209-212,4.基金项目
国家自然科学基金项目(30972454) （30972454）
深圳市重点实验室提升发展项目(CXB201005260068A) （CXB201005260068A）
深圳市科技计划重点项目(200801010) （200801010）
