安徽农业科学Issue(12)：7067-7068,7071,3.
HPLC法测定眼保Ⅰ号胶囊中葛根素和芍药苷的含量
Determination of Puerarin and Paeoniflorin in Yanbao I Capsule by HPLC
摘要
Abstract
[Objective] To establish the method for determining puerarin and paeoniflorin in Yanbao I capsule. [Method] The HPLC analysis used chromatography glass column Diamonsil C18(2) (4.6 mm ×250 mm, 5 μm) with the detection wavelength at 250 nm for puerarin and 230 nm for paeoniflorin and the column temperature was set at 25 ℃. Following the injection of sample solution, an elution was initiated at a flow rate of 1 ml/min with anhydrous methanol-water ( V/V,28:72) for Puerarin and acetonitrile-0. 1% phosphoric acid ( V/V, 14:86) as mobile phase. [Result] The linear range of puerarin was from 0.001 18 to 0.007 08 μg/ml (r=0. 999 9). The average recovery of sample was 102.4% with RSD of 1. 17% (n=6). The linear range of paeoniflorin was from 0.044 to 0.261 μg/ml (r =0.999 9). The average recovery of sample was 97. 8% with RSD of 1.71 % (n=6). In Yanbao 1 capsule, the content of puerarin and paeoniflorin could reach 0. 055 and 0.168 mg/g, respectively. [ Conclusion] The method is simple, accurate and repeatable, which is suitable for determining puerarin and paeoniflorin in Yanbao 1 capsule.关键词
HPLC/眼保Ⅰ号胶囊/葛根素/芍药苷Key words
HPLC/ Yanbao I capsule/ Puerarin/ Paeoniflorin分类
农业科技引用本文复制引用
谭家华,罗君,贺祝英,张建玲,于佳..HPLC法测定眼保Ⅰ号胶囊中葛根素和芍药苷的含量[J].安徽农业科学,2012,(12):7067-7068,7071,3.基金项目
贵州省中医药管理局中医药、民族医药科学技术课题研究项目(QZYY2010-28). （QZYY2010-28）
