安徽农业科学Issue(12)：7079-7082,4.
重组麻疯树核糖体失活蛋白表达及其体外抗肿瘤活性研究
Expression of Recombinant Curcin in Jatropha curcas L.and Its Antitumour Activity in vitro
徐单单 1张波 1黄亚东 2张齐好 2苏志坚 2许华 1郑青1
作者信息
- 1. 暨南大学药学院,广东广州510632
- 2. 暨南大学医药生物技术研究开发中心,广东广州510632
- 折叠
摘要
Abstract
[ Objective] To study the expression of recombinanl curcin in Jatropha curcas L. and its antilumour activity in vitro. [ Method] Curcin was cloned from the leaves of J. curcas,and the recombinanl vector pET-22b was established. The recombinant protein (29KD) was expressed in E. coli. [Result] Induced by 0.5 mM IPTG for 6 h at 30 ℃, expressing quantity of recombinant protein was about 20% of the total protein. Recombinant curcin was expressed in the form of inclusion body. After dissolution,renaturation and purification,purily of recombi-nant curcin reached 95.6%. [Conclusion] In vitro research showed that recombinant protein had significant proliferation inhibition effects on the A549 and HepG2 tumor cells. And their IC50 were 92. 246 and 691.42 μg/ml, respectively. But the recombinant protein had no significant inhibitory effects on Hela.关键词
麻疯树(Jatropha curcas L.)/curcin克隆/表达/纯化/抗肿瘤Key words
Jatropha curcas L. / Curcin/ Clone/ Expression/ Purify/ Anli-tumor分类
农业科技引用本文复制引用
徐单单,张波,黄亚东,张齐好,苏志坚,许华,郑青..重组麻疯树核糖体失活蛋白表达及其体外抗肿瘤活性研究[J].安徽农业科学,2012,(12):7079-7082,4.
