安徽农业科学Issue(12)：7085-7086,2.
HPLC法测定落葵果实中黄酮类化合物的含量
Determination of Flavonoids Content in Basella rubra L.Fruits by HPL
摘要
Abstract
[ Objective] To establish the determination method for flavonoids content in Basella rubra L. fruits. [ Method] With rutin as the reference substance, chromatographic column was Eclipse Zorbax XDB-C18(4.6 mm x 150 mm,5 μm) ; mobile phase was anhydrous metha-nol-water (V/V ,55 ;45) ,flow rale was 0. 8 ml/min; delection wavelength was 358 ntn; column temperature was room temperature. [ Result] Under the chromatographic conditions, regression equation of rutin standard curve was Y = 20.889X - 17. 147 ( R2 = 0.9982). Linear regression was good between the concentratian of 2.00 and 40. 0 μg/ml. The average recovery rate was greater than 96% and RSD was smaller than 2.0%. Flavonoids content in B. rubra fruits, was 179.0 μg/g by this method. [Conclusion] This method was simple,rapid,efficient, re-peatable,accurate and reliable,and was suitable for the content determination of flavonoids in B. rubra fruits.关键词
高效液相色谱/落葵果实/黄酮类化合物/芦丁Key words
HPLC/ Basella rubra L. fruits/ Flavonoids/ Rutin分类
轻工纺织引用本文复制引用
赵建芬,张素斌,严子军,王晓珊..HPLC法测定落葵果实中黄酮类化合物的含量[J].安徽农业科学,2012,(12):7085-7086,2.基金项目
肇庆市科学技术创新计划项目(2011G210). （2011G210）
