安徽农业科学Issue(12)：7155-7156,7239,3.
鼠李糖乳杆菌LT22发酵培养基优化
Optimization of the Growth Medium for a Lactobacillus rhamnosus LT22 Strain
摘要
Abstract
[Objective] To improve the concentration of Lactobaciilus rkamnosus LT22 in the fermentation broth. [Method] The effect of Plackett-Burman design on the influence factors of the medium was evaluated, and the three important factors were glucose, yeast extract and Tween 80. Then the central composite design and response surface analysis were used to determine the optimal levels of the important factors. [ Result] The best culture medium for Lactobacillus rhamnosus LT22 were 17 g glucose,12 g peptone,10 g beef extract,4. 8 g yeast extract, 3 g CH3COONa, 2 g diammonium hydrogen citrate, 0. 1 g MnSO4 · H2O, 0.6 g MgSO4 · 7H2O, 0.6 ml Tween 80 and 1 000 ml U2O. The amount of Lactobaciilus rhamnosus LT22 cultured by the optimized culture medium was 6.7 times that cultured by MRS. [ Conclusion] The results lay foundation for the animal microecological preparation of Lactobaciilus rhamnosus LT22.关键词
鼠李糖乳杆菌LT22/响应面分析/培养基优化Key words
Lactobaciilus rhamnosus LT22/ Response surface methodology/ Medium optimization分类
农业科技引用本文复制引用
谢为天,徐春厚,冯晓杰..鼠李糖乳杆菌LT22发酵培养基优化[J].安徽农业科学,2012,(12):7155-7156,7239,3.基金项目
广东省科技计划项目(2008B020800007). （2008B020800007）
