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大花萱草的花梗组织培养与快速繁殖

彭广霖 姜宁 潘仕梅 张志芬 史淑一

安徽农业科学2012,Vol.40Issue(17):9203-9205,3.
安徽农业科学2012,Vol.40Issue(17):9203-9205,3.

大花萱草的花梗组织培养与快速繁殖

Tissue Culture and Rapid Propagation of Pedicels of Hemerocallis hybrida

彭广霖 1姜宁 1潘仕梅 1张志芬 1史淑一1

作者信息

  • 1. 中国农业大学烟台研究院,山东烟台264670
  • 折叠

摘要

Abstract

[Objective] This study aimed to establish tissue culture and rapid propagation methods for pedicels of Hemerocallis hybrida. [ Method] Tender pedicels of dwarf H. hybrida were used as experimental materials to select callus induction, subculture and rooting medium for rapid propagation of H, hybrida. [Result] MS + 2.0 -3.0 mg/L of 6-BA +0.2 -0.3 mg/L of NAA, MS +1.0 -2.0 mg/L of 6-BA +0.1 -0.2 mg/L of NAA, MS and 1/2MS +0.2 mg/L of NAA were the appropriate media for callus induction, subculture and rooting, respectively. [Conclusion] The in vitro culture and clustered seedling rooting technology used in this study are effective methods for rapid propagation of H. hybrida, which provide technical reference for the industrialized production of H. hybrida.

关键词

大花萱草/花梗/离体培养/快速繁殖

Key words

Hemerocallis hybrid/Pedicel/ in vitro culture/ Rapid propagation

分类

农业科技

引用本文复制引用

彭广霖,姜宁,潘仕梅,张志芬,史淑一..大花萱草的花梗组织培养与快速繁殖[J].安徽农业科学,2012,40(17):9203-9205,3.

基金项目

中国农业大学(烟台)校级项目(yt2007.14). (烟台)

安徽农业科学

0517-6611

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