安徽农业科学2012，Vol.40Issue(17)：9251-9253,3.
HPD-400大孔树脂分离纯化栀子黄色素的工艺研究
Isolation and Purification of Gardenia Yellow Pigment by Macroporous Resin HPD-600
摘要
Abstract
[Objective] To study the absorption and purification conditions of Gardenia yellow pigment by macroporous resin. [ Method] The best macroporous resin was screened out from nine resins according to their adsorption and elution performance of Gardenia yellow pigment. The influence of temperature,acidity,etc. on the absorption and desorption of HPD-400 was investigated to explore its best isolation and purification conditions. [Result] HPD-400 showed the largest adsorption capacity,its best adsorption conditions were 25 ℃ temperature, pH 3.0 and the concentration of crude extracts A440nm= 0.590; the best elution conditions were 70% ethanol concentralion and 25 ℃ temperature. [Conclusion ] HPD-400 shows the best isolation and purification effects of Gardenia yellow pigment,thus can be used in industrialized production.关键词
栀子黄色素/大孔吸附树脂/吸附/解吸Key words
Gardenia yellow pigment/ Macroporous resin/ Absorption/ Desorption分类
农业科技引用本文复制引用
高丽,邓青云,姜益泉,李国元,张昭..HPD-400大孔树脂分离纯化栀子黄色素的工艺研究[J].安徽农业科学,2012,40(17):9251-9253,3.基金项目
湖北省自然科学基金重大项目,鄂财企发[2009]91号第931号. （）
