安徽农业科学2012，Vol.40Issue(17)：9276-9277,9279,3.
仔猪大肠杆菌·C型产气荚膜梭菌菌液的培养工艺
Study on Culture Technology of Escherichia coli and Clostridium Perfringens Type C of Piglets
摘要
Abstract
[Objective] The aim was to study the culture technology of Escherichia coli and clostridium perfringens type C of piglets. [ Method ]Effects of different culture time, culture temperature and inoculation dosage on the cilia expression of E. coli and toxin production of clostridium perfringens type C were studied respectively, [Result] The results showed that the content of fimbriae antigen was higher when inoculation dosage of E. coli was i%-5% ,and cultured at 36 -37 ℃ for 7 h. There would produce huge amounts lethal toxin when inoculation dosage of clostridium perfringens type C was 1% -2% ,and cultured at 35 ℃, for 16 -20 h. [Conclusion] The study provides a basis for development with good effect prevention vaccine of red scour of piglets and white scour of piglets.关键词
大肠杆菌/C型产气荚膜梭菌/培养工艺/抗原含量/仔猪红痢/仔猪黄痢Key words
Escherichia coli/ Clostridium perfxingens type C/ Culture technology/ Antigen content/ Red scour of piglets/ White scour of piglets分类
农业科技引用本文复制引用
刘泽文,袁芳艳,周丹娜,梁望旺,段正赢,杨克礼,徐涤平,田永祥..仔猪大肠杆菌·C型产气荚膜梭菌菌液的培养工艺[J].安徽农业科学,2012,40(17):9276-9277,9279,3.基金项目
湖北省科技厅引导项目(2006AA201C25) （2006AA201C25）
湖北省科技创新中心项目(2007-620-001-03). （2007-620-001-03）
