安徽农业科学2012，Vol.40Issue(17)：9332-9335,4.
葱蝇Dorsal基因的克隆·表达及生物信息学分析
Cloning,Expression and Bioinformatic Analysis of Dorsal Gene in Delia antiqua
摘要
Abstract
[Objective] The aim was to explore the relationship between the Dorsal and diapause development of Delia antiqua. [Method] The full-length cDNA of Dorsal in D. antiqua was cloned through RACE. The similarity comparison among deduced amino acid sequence of DaDor-sal cDNA and the Dorsals of other 14 insect species was compared, and the phylogenetic analysis of these Dorsals was conducted. The expression level of Dorsal gene in winter-,summer- and non-diapaueing pupae was analyzed. [ Result] The full-length of Dorsal cDNA sequence, 2 412 bp,was obtained with ORF 1 974 bp, which coded 657 amino acids with predicted Mw 72. 9 kDa and Pl 8. 5. The result of similarity comparison indicated that the DaDorsal was most related to those of Drosophlia. melanogaster and Drosophlia pseudoobscura. The result of semi-quantitative RT-PCR analysis showed the expression level of Dorsal gene increased in characteristic duration of winter-, summer- and non-dia-pausing pupae,especially at the late diapause,which might imply its relationship to D, antiqua diapause and development. [Conclusion] The study lays the foundation for further study on gene function of Dorsal in insect diapause and development.关键词
Dorsal基因/葱蝇/RACE/序列分析/系统发育树/半定量分析Key words
Dorsal/ Delia antiqua/RACE/ Sequence analysis/ Phylogenetic analysis/ Semi-quantitative RT-PCR分类
农业科技引用本文复制引用
魏慧敏,陈斌,任爽..葱蝇Dorsal基因的克隆·表达及生物信息学分析[J].安徽农业科学,2012,40(17):9332-9335,4.基金项目
国家自然科学基金项目(30870340,31071968) （30870340,31071968）
重庆市教委科学技术研究项目(KJ100620) （KJ100620）
重庆师范大学校级基金重点项目(2011XLZ12). （2011XLZ12）
