安徽医科大学学报2012，Vol.47Issue(8)：924-927,4.
1,25-二羟维生素D3下调Toll样受体7在树突状细胞中的表达
The down-regulation of TLR7 expression in dendritic cells by 1,25-dihydroxyvitamin D3
摘要
Abstract
Objective To investigate the influence of 1,25-dihydroxyvitamin D3 on dendritic cell( DC ) of pheno-type, function and TLR7 expression, to explore the possible role of TLR7 in the procedure of the inducement of tolerance DC by 1,25-dihydroxyvitamin D3. Methods DC were harvested by differentiating mouse borrow cells in vitro. The influence of 1, 25-dihydroxyvitamin D3 on phenotype and function of DC was determined by flow cytome-try and mixed lymphocyte reactions. The expression of TLR7 on 1,25-dihydroxyvitamin D3-induced DC was determined by semi-quantitative RT-PCR. Results Treated by 1, 25-dihydroxyvitamin D3, DC had significantly lower fluorescence intensity of CD80 and MHC II ( P < 0. 05 ), and DC ability to stimulate T cells was significantly lower than that of the control( P <0. 05 ). Compared to the control, 1,25-dihydroxyvitamin D3 could obviously down-regulate the expression of TLR7( P < 0. 01 ). Conclusion 1, 25-dihydroxyvitamin D3 can inhibit the maturation of DC, and may make DC tolerant by down-regulating the expression of TLR7.关键词
树突状细胞/1,25-二羟维生素D3/Toll样受体7Key words
dendritic cell/l ,25-dihydroxyvitamin D3/ Toll-like receptor 7分类
医药卫生引用本文复制引用
刘飞,杨明珍,李庆生,宋玲,夏雷鸣..1,25-二羟维生素D3下调Toll样受体7在树突状细胞中的表达[J].安徽医科大学学报,2012,47(8):924-927,4.基金项目
安徽省自然科学基金项目(编号:1208085MH154) （编号:1208085MH154）
