安徽医科大学学报2012，Vol.47Issue(8)：931-935,5.
UUO大鼠肾脏CNP表达特点及其对ECM代谢的调控效应
Expressive characterization of CNP and its modulation to ECM metabolism in the obstructed kidney
摘要
Abstract
Objective To explore the expressive characterization of C-type natriuretic peptide ( CNP ) and its modulation of extracellularmatrix( ECM ) metabolism in the kidney of unilateral ureteral obstruction( UUO ) model rats. Methods Twenty four healthy male Wistar rats were randomly divided into two groups: UUO group and sham group. UUO model was established and all rats were killed at the 1st day and the 3th month. In both groups, the proteinuria and plasma levels were examinated. Renal morphological changes were observed by HE staining. Expression of CNP, MMP-2, MMP-9, TIMP-1, Col-IV were observed by immunohistochemistry, real time-PCR. Results At the 3 th month, compare with the sham group, proteinuria levels, albumin, serum creatinine, blood urea nitrogen levels increased in the UUO group( P < 0. 05 ). Immunohistochemical assay and real time-PCR revealed that the expression of CNP, MMP-2, MMP-9 decreased and TIMP-1, Col-IV increased in the renal tissue of UUO rats( P < 0. 05 ). Conclusion The CNP expression decreases in the kidney of UUO rats, which may be caused the imbalance of ECM and tububinterstitial fibrosis.关键词
C型利钠肽/细胞外基质/肾小管间质纤维化Key words
C-type natriuretic peptide/ extracellularmatrix/ tubular-interstitial fibrosis分类
医药卫生引用本文复制引用
汪婧,胡鹏,胡波..UUO大鼠肾脏CNP表达特点及其对ECM代谢的调控效应[J].安徽医科大学学报,2012,47(8):931-935,5.基金项目
国家自然科学基金(编号:81000306) （编号:81000306）
安徽医科大学校科研基金(编号:2010xkjo55) （编号:2010xkjo55）
安徽医科大学第一附属医院博士启动基金(编号:2009kj02) （编号:2009kj02）
