安徽医科大学学报2012，Vol.47Issue(8)：940-943,4.
5,7,3'-三乙酰橙皮素在大鼠体内的药代动力学研究
Pharmacokinetics of 5,7,3'-triacetyl hesperetin in rats
摘要
Abstract
Objective To develop an HPLC detection for the quantification of 5 ,7,3'- triacetyl hesperetin( TAHP ) in rat plasma and study the pharmacokinetics in rats. Methods A C18 reversed-pase chromatography column was used, and the mobile phase of methyl alcohol-0. 5 % glacial acetic acid was applied to perform gradient dilution at flow rate of 1. 0 ml/min. The detection wavelength was set at 288 nm and the column temperature was 40?. A single oral dose of TAHP was performed in SD rats at 25 ,50,100 mg/kg. Plasma sample was prepared after hydrolysis with p-glucuronidase and determined by HPLC. Plasma concentration-time data were analyzed by DAS 2. 0. Results The calibration curve was linear within 0. 05～25 100/ml( r = 0. 999 7 ). The recoveries were more than 80% , with the intraday- and interday-RSDs less than 10%. The main pharmacokinetic parameters( 25, 50, 100 mg/kg) were Tmax(h): 1.30 ±1.13, 1.80±1.30, 3. 17 ±0.41; Cmax( mg/L): 0.90±0. 13, 1.65± 0.34, 4. 27 ±0.84; AUC(0-t)( mg/h · L): 7. 57± 1. 51, 17. 84± 1. 81, 61. 67±19. 04, respectively. Conclusion The method is proved to be convenient, rapid, accurate and specific and has been successfully applied to pharmacokinetic study in rats. The plasma drug concentration-time data curve of the active metabolite of TAHP conforms to two compartments model of the first level absorption.关键词
5,7,3'-三乙酰橙皮素/橙皮素/高效液相色谱法/药动学Key words
5 ,7,3'-triacetyl hesperetin/ hesperetin/ HPLC/ pharmacokinetics分类
医药卫生引用本文复制引用
秦娣,李俊,李荣,陈昭琳,黄成,黄艳..5,7,3'-三乙酰橙皮素在大鼠体内的药代动力学研究[J].安徽医科大学学报,2012,47(8):940-943,4.基金项目
国家重大基础研究前期(973前期)研究专项(编号:2002CCC02900) （973前期）
