草业科学2012，Vol.29Issue(6)：918-923,6.
盐生植物盐地碱蓬质膜Na^＋/H^＋逆向转运蛋白基因片段的克隆及其序列分析
Cloning and sequence analysis of a plasma membrane Na^＋/H^＋ antiporter fragment from halophyte Suaeda salsa
摘要
Abstract
To investigate long-distance transport of Na^＋ in Suaeda salsa, degenerate primers were designed based on the conserved sequences of the SOS1 genes from other plants. Total RNA was extracted from the roots of S. salsa and the SOS1 gene fragment was obtained by reverse transcription polymerase chain reac-tion （RT-PCR） aiming to provide the foundation for cloning full-length SOS1 gene and expression regula-tion. The sequencing result showed that the SOS1 gene fragment （736 bp） from S. salsa encoded 224 ami-no acids. Homology comparison showed that the SOS1 gene fragment shared over 740% nucleotide and 61% amino acid similarity to SOSI fragments from other plants.关键词
盐地碱蓬/SOS1基因/克隆/序列分析Key words
Suaeda salsa/SOS1 gene/cloning/sequence analysis分类
农业科技引用本文复制引用
王生银,马清,王锁民..盐生植物盐地碱蓬质膜Na^＋/H^＋逆向转运蛋白基因片段的克隆及其序列分析[J].草业科学,2012,29(6):918-923,6.基金项目
国家自然科学基金 （）
教育部博士学科点专项科研基金 （）
