草业科学2012，Vol.29Issue(6)：924-930,7.
淮阴苜蓿SSR指纹图谱的构建
Construction of fingerprinting map based on SSR for Medicago sativa Huaiyin
摘要
Abstract
In the present study, the genetic diversity of alfalfa was investigated using SSR molecular makers and optimum number of samples was determined using different sampling strategies. Based on the opti- mum number of samples with the screened SSR primers, the fingerprint of Huaiyin alfalfa was built and the varieties of Huaiyin alfalfa were identified. Genomic DNA from 320 strains of Huaiyin alfalfa was set to six treatments （alfalfa DNA samples randomly obtained from a mixture of equal amount of 10, 20, 30, 40, 50 and 60 plants , respectively）. Each treatment has eight repeats. Genetic diversity analysis was con-ducted using four pairs of SSR primers. The results showed that the genetic diversity index of the treat-ment of andom DNA mixture from 40 individuals was stable and the fingerprint of Huaiyin alfalfa can represent the genetic diversity of Huaiyin alfalfa groups. Fifteen alfalfa materials were tested with the treatment mentioned above and the results showed that the SSR fingerprinting could be used for the identi-fication of varieties in Huaiyin alfalfa groups.关键词
淮阴苜蓿/取样策略/品种鉴定/遗传多样性/指纹图谱Key words
Medicago satiua Huaiyin/sample size/identification/genetic diversity/fingerprint分类
农业科技引用本文复制引用
张栋,魏臻武,武自念,屠德鹏,李伟民..淮阴苜蓿SSR指纹图谱的构建[J].草业科学,2012,29(6):924-930,7.基金项目
国家863计划项目“高产、多抗、优质苜蓿新品种分子聚合育种” （）
国家自然科学基金项目“豆科模式植物蒺藜苜蓿种子产量相关性状的比较基因组学研究” （）
