草业科学2012,Vol.29Issue(6):943-949,7.
20份马蹄金种质资源遗传多样性RAPD和SRAP分析
Genetic diversity analysis of 20 Dichondra repens germplasm by RAPD and SRAP
摘要
Abstract
Genetic diversity of 20 Dichondra repens wild germplasm collected from different location in southwest China,compared with an imported American variety(Common),were analyzed by RAPD and SRAP markers.The results showed that 20 RAPD primers produced 189 bands,including 130 polymorphic bands;the average percentage of polymorphic bands was 68.78%.22 pairs of SRAP primers produced 242 bands,including 175 polymorphic bands;the average percentage of polymorphic bands was 72.31%.The 21 germplasm could be clustered into either two groups based on RAPD markers as well as SRAP markers.Moreover,the tested germplasm with same origin or similar morphology frequently clustered into the same group and showed a nice geographical distribution and relationships.There was significant positive correlation between RAPD and SRAP(r0.01=0.910),which indicated that the two methods were high consistant and reliable for detecting genetic diversity and genetic relationships in D.repens germplasms,though some germplasm were clustered into different groups between the two markers.关键词
马蹄金/RAPD标记/SRAP标记/遗传多样性Key words
Dichondra repen/RAPD/SRAP/genetic polymorphism分类
农业科技引用本文复制引用
付薇,周玉锋,于友民,王小利,张建波..20份马蹄金种质资源遗传多样性RAPD和SRAP分析[J].草业科学,2012,29(6):943-949,7.基金项目
贵州省“十一五”重大专项“草坪工厂化生产技术研究与产业化示范推广” ()
四川省科技厅应用基础研究专项基金资助项目 ()