草业学报2012，Vol.21Issue(3)：287-293,7.
苜蓿皂甙对高脂血症大鼠胆固醇代谢及肝脏ACAT-2和HMG-CoAr基因表达的影响
Influence of alfalfa saponins on cholesterol metabolism and the expressions of ACAT-2 and HMG-CoAr in the liver of hyperlipidemia rats
摘要
Abstract
The effect of alfalfa saponins on cholesterol metabolism and the expressions of ACAT-2 and HMG-CoAr in the liver of hyperlipidemia rats was investigated. Forty healthy male Sprague Dawley (SD) rats were randomly divided into four groups: normal (control, A), hyperlipidemia model (B), alfalfa saponins prevention group (C) , alfalfa saponins treatment group (D). Except for the controls, rats were fed a high-fat diet for eight weeks. Alfalfa saponins were added by gavage to group C from the first week, and to group D from the fifth week. Body weight, liver index and serum lipid level of the rats were measured. Protein and mRNA expressions of ACAT-2 and HMG-CoAr were measured by enzyme linked immunosorbent assay (ELISA) and real-time PCR methods respectively. 1) The body weight, liver index and serum lipid level of hyperlipidemia rats were significantly reduced, and the fatty degeneration and steatosis in the liver were ameliorated by alfalfa saponins. 2) The protein and mRNA expressions of ACAT-2 in groups C and D were significantly lower than those in group B(P<0. 01), but there was no significant difference for HMG-CoAr. In conculsion, alfalfa saponins prevented hyperlipidemia by reducing the expression of hepatic ACAT-2, and by inhibiting the absorption of exogenous cholesterol.关键词
苜蓿皂甙/SD大鼠/高脂血症/胆固醇代谢/ACAT-2/HMG-CoArKey words
alfalfa saponins/ SD rats/ hyperlipidemia( cholesterol metabolism/ ACAT-2/ HMG-CoAr分类
农业科技引用本文复制引用
王先科,史莹华,王成章,陈明亮,袁德地..苜蓿皂甙对高脂血症大鼠胆固醇代谢及肝脏ACAT-2和HMG-CoAr基因表达的影响[J].草业学报,2012,21(3):287-293,7.基金项目
国家牧草产业技术体系建设项目(nycytx-37)和国家"十二五"科技支撑计划项目(2011BAD17B04)资助. （nycytx-37）
