重庆医学2012，Vol.41Issue(19)：1897-1899,3.DOI:10.3969/j.issn.1671-8348.2012.19.001
联合偶合概率与似然比率在炭化骨骼个人识别中的计算及分析
Analysis of random match probability ratio and likelihood ratio in adult burning bone
摘要
Abstract
Objective To analyse random match probability ratio(RMP) and likelihood ratio(LR) in adult burning bone. Meth-ods 2 adult femoral bone,female and male in half,were incinerated at 300°C for 1 hour in normal pressure,then grinded to be bone meal in the liquid nitrogen before decalcification by ethylenediamine tetraacetic acid(EDTA). The deoxyribonucleic acid(DNA) was extracted by classical phenol and chloroform,subsequently the DNA was five-fluorescent labeled,amplificated by PCR,and the data was collected and analyzed by using genetic analyzer. Results The TDP value was 0. 999 999 980 3,and that of the female was> 0. 999 999 999 999 99. The RMP value of the male was 2. 322 5×10-12 ,and that of the female was 4. 931 3×10-24. The LR of the male was 4. 305 7×1011 , and the female was 2. 027 9× 1023. Conclusion The DNA can be extracted from the bone burned at 300°C ,and the remaining gene locus atlas comparion, the TDP, RMP, LR value can be used for individual identification.关键词
法医物证学/个人识别/DNA检验/烧骨Key words
forensic biology/individual identification/DNA test/burned bone引用本文复制引用
徐国昌,齐静,任甫,刘海东..联合偶合概率与似然比率在炭化骨骼个人识别中的计算及分析[J].重庆医学,2012,41(19):1897-1899,3.基金项目
辽宁省百千万人才工程培养经费资助项目(2010921042). （2010921042）
