重庆医学2012，Vol.41Issue(19)：1906-1908,3.DOI:10.3969/j.issn.1671-8348.2012.19.004
电离辐射致线粒体DNA4977bp缺失与肿瘤细胞放射敏感性关系的初步研究
Preliminary study of relationship between radiation-induced mitochondrial DNA 4 977 bp deletion and radiosensitivity of human tumor cell lines
摘要
Abstract
Objective To explore the potential relationship of the radiation-induced mitochondrial DNA A 977 base pairs(mtDNA A 977 bp) deletion and the radiosensitivity of tumor cell lines. Methods The surviving fraction(SF) and the ratio of mtDNA A 977 bp deletion were determined by clone forming assay and nested polymerase chain reaction(PCR) technique respectively after tumor cells of Hela, HepG2 ,EC-9706 and PC-3 were irradiated by various doses of X-ray radiation from 0 Gy to 8 Gy. Results The de-scending order of the four tumor cell lines radiosensitivities were the Hela, HepG2 ,EC9706 and PC-3 cells. The radiation-induced mtDNA A 977 bp deletion were detected after 1 Gy irradiated in Hela cells and 2 Gy irradiated in HepG2 ,EC-9706 and PC-3 cells. The deletion rate of the four tumor cell lines had no significant difference at 1 Gy(P>0. 05) ;but the deletion rate of Hela cells was significantly higher than that of the other tumor cell lines at 2 Gy(P<0. 01) ;at A Gy and 8 Gy,the deletion rate of Hela cells was significantly higher than that of the other tumor cell lines(P<0. 05) ,and the deletion rates of HepG2 and EC-9706 cells were high-er significantly than that of PC-3 cells(P<0. 01). There were negative corralations between the SF and the deletion rates in Hela, HepG2 and EC-9706 cells(r= - 0. 951,P<0. 05;r=-0. 976,P<0. 01;r= -0. 986,P<0. 01). Conclusion The ratio of mtDNA A 977 bp deletion may be an biological marker to predict the radiosensitivity of tumor cells.关键词
DNA,线粒体/克隆形成法/巢式PCR/辐射耐受性Key words
DNA,mitochondrial/clone forming assay/nested polymerase chain reaction/radiation tolerance引用本文复制引用
荣庆林,刘莉,王芹,刘强,陈艳芳,姜文华,李小东..电离辐射致线粒体DNA4977bp缺失与肿瘤细胞放射敏感性关系的初步研究[J].重庆医学,2012,41(19):1906-1908,3.基金项目
天津市自然科学基金资助项目(07JCYBJC09200). （07JCYBJC09200）
