重庆医学2012，Vol.41Issue(19)：1951-1953,1956,4.DOI:10.3969/j.issn.1671-8348.2012.19.021
高渗氯化钠羟乙基淀粉40注射液对非控制出血性休克大鼠凝血功能及脂质过氧化的影响
Influence of hypertonic sodium chloride hydroxyethyl starch 40 on blood coagulation and lipid peroxidiation for uncontrolled hemorrhagic shock in rats
摘要
Abstract
Objective To investigate the influence of hypertonic sodium chloride hydroxyethyl starch 40(HHS40)on blood coag-ulation and lipid peroxidiation for uncontrolled hemorrhagic shock in rat. Methods Rat models of uncontrolled hemorrhagic shock were established using the modified Capone method. SD rats(n = 30) were randomly divided into 3 groups, normal control group (group NC),normal saline group(group NS) and HHS40 group. The animals were inflicted with a sharp resection of 15% of the tail to initiate the hemorrhage. When the blood pressure(MAP) was decreased to 40 mm Hg,the animals received the corresponding infusion respectively for 1 h when the MAP was increased to 50 mm Hg. The blood samples were collected at various time points to measure Hct,PT, APTT, FIB, content of malondialdehyde(MDA) and the activity of superoxide dismutase(SOD). Results Hct was decreased obviously at T2 - T1 than at T1 in HHS40 group and group NS(P<0. 05). HHS40 could significantly enhanced Hct of uncontrolled hemorrhagic shock rats at T2 - T4 as compared with those in group NS(P<0. 05) ,meanwhile it also obviously de-creased plasma MDA of the shock rats(P<0. 05). Pt and APTT were increased evidently at T2 - T4 than at T1 in HHS40 group and group NS(P<0. 05). PT and APTT were decreased obviously in HHS40 group than those in group NS at T2 - T4 (P< 0. 05). But FIB had no obvious difference between two groups(P>0. 05). SOD was decreased obviously and MDA was increased evidently at T2 - T, than at T1 in HHS40 group and group NS(P<0. 05). MDA was lower obviously and SOD was higher evident-ly in HHS40 group than those in group NS at T2 - T4. Conclusion HHS40 can decrease hemodilution and oxygen free radicals. There is effect on blood coagulation, but exceed compensatory airframe range not yet. Therefore, it may be beneficial for the treat-ment of uncontrolled hemorrhagic shock.关键词
高渗氯化钠羟乙基淀粉40注射液/休克,出血性/血液凝固/脂质过氧化作用Key words
hypertonic sodium chloride hydroxyethyl starch 40 injection/ shock, hemorrhagic/ blood coagulation/ lipid peroxidi-ation引用本文复制引用
陈慧,朱昭琼,王钊,韩明..高渗氯化钠羟乙基淀粉40注射液对非控制出血性休克大鼠凝血功能及脂质过氧化的影响[J].重庆医学,2012,41(19):1951-1953,1956,4.基金项目
贵州省科学技术基金资助项目[黔科合J字(2009)2183号]. （2009）
